1. Animal immunity and preparation of serum IgG.
2. Immunohistochemical blocking, ELISA and other immunoassay blocking and the use of protective agents.
Immunohistochemistry: Seal with 5-10% normal donkey serum (1:10-1:20-fold dilution) and incubate at room temperature for 5-10 minutes. Remove serum, do not wash, add appropriate proportion of diluted primary antibody or primary antibody working solution, incubate at 37℃ or room temperature at 4℃ overnight. Cell smear: Sealed with 5-10% normal donkey serum (1:10-1:20-fold dilution), diluted with PBS (0.01mol/L pH7.4), a small amount of drip: Mote over cell smear ELISA: 1:100 fold dilution was used with PBS (0.01mol/L pH7.4) and the sealing time was 37℃ for an hour.
Store at 2-4℃ for 2-3 weeks; -20℃ for 5 years; Avoid repeated freeze-thaw cycles.
Normal donkey serum; The ass/donkey serum
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