worldwide@absin.cn
  • Sign in or Register
    My account Recent orders Wish list Address management

    Sign out

  • Cart 0
    The latest additions

      0 items in total

      Subtotal $0.00

      Go to cart
    • English
      Chinese
    爱必信(absin)官网
    爱必信(absin)官网

    000000 Citations

    Home
    Products
    Promotions
    Resources
    Product ApplicationFAQsNewsCitationsElectronic DocumentsCalculation Tools
    About us
    Contact us
    worldwide@absin.cn
    • Sign in or Register
      My account Recent orders Wish list Address management

      Sign out

    • Cart 0
      The latest additions

        0 items in total

        Subtotal $0.00

        Go to cart
      • English
        Chinese
      爱必信(absin)官网
      爱必信(absin)官网

      000000 Citations

      Home
      Products
      Promotions
      Resources
      Product ApplicationFAQsNewsCitationsElectronic DocumentsCalculation Tools
      About us
      Contact us
      Home
      abs553805

      Rat IL-10 High Sensitivity ELISA Kit

      Product Image
      • Product Details
      • FAQ
      • Documents

      Detection Principle:

      This kit uses double antibody sandwich ELISA technology. Specific anti rat IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

      Detection Type: double antibody sandwich method

      Form: Pre coated 96 well plate

      Test Sample Type: cell supernatant, serum, plasma

      Loading Amount: 100 μ L

      Kit Components: a copy of pre coated 96 well plate, standard, biotin labeled IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

      Sensitivity: 0.22 pg/ml

      Detection Range: 6.25-400 pg/ml

      Recovery Range: 94-115%

      Storage Method: 2-8 ℃

      Standard Curve:

      Background:

      Interleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
      Dear customer, if you have any questions or want to know about our products, you can click the "I want to ask" button to fill in your questions. Unsuccessful submission? Please contact worldwide@absin.cn.
      Ask questions
      • Publication date
      • Impact factor
      Search
      • The batch number you entered does not exist. Please input the correct batch number information
      • Datasheet product specification
      • Products
      • Resources
      • Product Application
      • Calculation Tools
      • FAQs
      • Citations
      • Electronic Documents
      • About us
      • Company Profile
      • Ordering Tools
      • Customer Support
      • Contact us
      Email: worldwide@absin.cn
      picture invalid picture invalid
      ICP:16001690 Absin Bioscience Inc.All rights reserved.