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Rat IFN-γ ELISA Kit
IFNG, Interferon γ
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Detection Principle:
This kit uses double antibody sandwich ELISA technology. Specific anti rat ifn-γ Antibody was pre coated on a high affinity microplate. Add standards, samples to be tested and biotinylated detection antibodies into the wells of the enzyme plate. After incubation, ifn-&gamma in the samples; Bound to solid-phase antibody and detection antibody. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color response and ifn-&gamma in the sample; Is proportional to the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).
Detection Type: double antibody sandwich method
Form: pre coated 96 well plate
Detection Sample Type: cell supernatant, serum, plasma
Loading Volume: 100ul
Kit Components: Pre coated 96 well plate, standard, rat ifn-γ One copy of detection antibody, dilution buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions
Sensitivity: 0.54 pg/ml
Detection Range: 31.25 pg/ml - 2000 pg/ml
Recovery Range: 87-112%
Storage Method: 2-8 ℃
Standard Curve
Background:
γ Interferon (IFN-γ) is a soluble dimeric cytokine and is the only member of interferon II. It is mainly secreted by natural killer (NK) and natural killer T (NKT) cells and plays a role in innate immunity; It is secreted by CD4 Th1 and CD8 cytotoxic T cells during antigen-specific immunity. IFN-γ Or type II interferons play an important role in innate and adaptive immunity against virus, some bacteria and protozoan infections. IFN-γ It is an important activator of macrophages and an inducer of major histocompatibility complex type II (MHC II) expression. IFN-γ The abnormal expression of Bcl-2 is associated with many autoinflammatory and autoimmune diseases. In addition to directly inhibiting viral replication, IFN-γ Its importance to the immune system is more reflected in its immune stimulation and immune regulation functions. Ifn-&gamma in many different pathological conditions, including infection, autoimmune diseases, transplant rejection, allergic reactions, diabetes, etc; It can be used as a disease marker.
This kit uses double antibody sandwich ELISA technology. Specific anti rat ifn-γ Antibody was pre coated on a high affinity microplate. Add standards, samples to be tested and biotinylated detection antibodies into the wells of the enzyme plate. After incubation, ifn-&gamma in the samples; Bound to solid-phase antibody and detection antibody. After washing to remove unbound material, horseradish peroxidase labeled streptavidin HRP was added. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color response and ifn-&gamma in the sample; Is proportional to the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).
Detection Type: double antibody sandwich method
Form: pre coated 96 well plate
Detection Sample Type: cell supernatant, serum, plasma
Loading Volume: 100ul
Kit Components: Pre coated 96 well plate, standard, rat ifn-γ One copy of detection antibody, dilution buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions
Sensitivity: 0.54 pg/ml
Detection Range: 31.25 pg/ml - 2000 pg/ml
Recovery Range: 87-112%
Storage Method: 2-8 ℃
Standard Curve
Background:
γ Interferon (IFN-γ) is a soluble dimeric cytokine and is the only member of interferon II. It is mainly secreted by natural killer (NK) and natural killer T (NKT) cells and plays a role in innate immunity; It is secreted by CD4 Th1 and CD8 cytotoxic T cells during antigen-specific immunity. IFN-γ Or type II interferons play an important role in innate and adaptive immunity against virus, some bacteria and protozoan infections. IFN-γ It is an important activator of macrophages and an inducer of major histocompatibility complex type II (MHC II) expression. IFN-γ The abnormal expression of Bcl-2 is associated with many autoinflammatory and autoimmune diseases. In addition to directly inhibiting viral replication, IFN-γ Its importance to the immune system is more reflected in its immune stimulation and immune regulation functions. Ifn-&gamma in many different pathological conditions, including infection, autoimmune diseases, transplant rejection, allergic reactions, diabetes, etc; It can be used as a disease marker.
Tips : This product is for research use only. Not for use in diagnostic prodcedures.