Rat IFN-γ ELISA KIT

Detection principle:

In this experiment, a double antibody sandwich was used ELISA Law. anti-rat IFN-γ Monoclonal antibody coating on microplates, samples and standards IFN-γ It will bind to the antibody immobilized on the plate, and free components will be washed away; Add biotin-labeled anti-rat IFN-γ Multiple antibodies, and those bound to microplates IFN-γ By binding, free components are washed away; Streptavidin-labeled horseradish peroxidase, which specifically recognizes biotin, is then added to form a complex. After washing away free components, a substrate solution (developer) is added, and the solution gradually turns blue. After adding a stop solution, the solution turns yellow and stops changing. Absorbance was determined with a microplate reader.

Test Type: double antibody sandwich method

Format: pre-wrapped 96 orifice plate

Test Sample Type: Cell supernatant, serum, plasma

Sample Load: 100ul

Kit Components:

pre-wrapped 96 Well plates, standards, anti-rat IFN-γ Detection antibody, dilution buffer, chromogenic solution ( A 、 B ), washing solution, termination solution, SA-HRP One copy of the sealing film and the instruction manual.

Sensitivity: 15.6 pg/mL

Detection range: 39.1 - 2,500 pg/mL

Recovery range: 89.0-118.9%

Storage method: 2-8℃

Standard graph

Background:

IFN-γ (Interferon -γ ) is a typical pro-inflammatory cytokine, produced by various immune cells under inflammatory conditions, especially by T cells and NK Cell production. It promotes Th1 Cell development and activation, chemical attraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and B Immunoglobulin class switching in cells plays a key role in host defense. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ By promoting regulation T Cell development and inhibition Th17 Cells differentiate to act as anti-inflammatory mediators. IFN-γ The dimer passes through two IFN-γR1 and two IFN-γR2 The receptor complex of the subunit signals.