Rat IFN-γ ELISA KIT

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti rat IFN-γThe monoclonal antibody was coated on the microplate, and IFN-γ in samples and standards; It will bind to the antibody fixed on the plate, and the free components will be washed away; Biotin labeled anti rat IFN-γ was added; Polyclonal antibody, and IFN-γ bound to the microplate; Binding, free components are washed away; The streptavidin labeled horseradish peroxidase, which specifically recognizes biotin, is added to form a complex. After washing away the free components, the substrate solution (chromogenic agent) is added, and the color of the solution gradually turns blue. After adding the stop solution, the solution turns yellow and stops changing. The absorbance was measured with a microplate reader.

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Detection Sample Type: cell supernatant, serum, Plasma

Loading Amount: 100ul

Kit Components:

Precoated 96 well plates, standards, anti rat IFN - γ One copy of detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions

Sensitivity: 15.6 pg/ml

Detection Range: 39.1 - 2500 pg/ml

Recovery Range: 89.0-118.9%

Storage Method: 2-8 ℃

Background:

IFN-γ (interferon -γ)It is a typical proinflammatory cytokine produced by a variety of immune cells under inflammatory conditions, especially by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen-presenting molecules, and immunoglobulin class switching in B cells. It also has antiviral, antiproliferative and apoptotic effects. In addition, IFN-γ Acts as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. Ifn-γ Dimers pass through two ifn-γ R1 and two ifn-γ The receptor complex of the R2 subunit signals.