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1. Animal immunity and preparation of serum IgG. 2. Immunohistochemical blocking, ELISA and other immunoassay blocking and use of protective agents. Can not be used as medical products. No inactivation, no sterilization. It must be inactivated and sterilized if used for cell culture. No preservatives.
Immunohistochemistry: Sealed with 5-10% normal rabbit serum (1:10-1:20-fold dilution) and incubated in greenhouse for 5-10 minutes. Remove serum, do not wash, add appropriate dilution of primary or secondary antibody working solution and incubate overnight at 37ºC or 4ºC in a greenhouse.
Cell smear: sealed with 5-10% normal rabbit serum (1:10-1:20-fold dilution), diluted with PBS (0.01mol/L pH7.4), a small amount of drip: the cell smear can not pass.
ELISA: 1:100 fold dilution, using PBS dilution (0.01mol/L pH7.4) sealing time: 37ºC for 1 hour.
Store at -20℃ for 5 years.
Normal rabbit serum (non-medical) for blocking; Rabbit serum
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