Rabbit anti-F4/80 Polyclonal Antibody

Adhesion G protein-coupled receptor E1; EGF-like module receptor 1; EMR1 hormone receptor; Gpf480; Adgre1

Reactivity：

Mouse, Rat

Application：

WB, IHC-P

Host：

Rabbit

Clonality：

Monoclonal Antibody

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Tips : This product is for research use only. Not for use in diagnostic prodcedures.

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Experimental result diagram

WB result of F4/80 Rabbit mAb Primary antibody: F4/80 Rabbit mAb at 1/1000 dilution Lane 1: NIH/3T3 whole cell lysate 20 µg Lane 2: RAW264.7 whole cell lysate 20 µg Negative control: NIH/3T3 whole cell lysate Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 97kDa Observed MW: 300kDa Exposure time: 180s

IHC shows positive staining in paraffin-embedded mouse liver. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse colon. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse spleen. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse stomach. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embedded mouse skeletal muscle. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat liver. Anti-F4/80 antibody was used at 1/500 dilution, followed by a HRP Polymer for Mouse & Rabbit IgG (ready to use). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.