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Rabbit anti-CD45 Polyclonal Antibody








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WB result of CD45 Rabbit mAb Primary antibody: CD45 Rabbit mAb at 1/2500 dilution Lane 1: MCF7 whole cell lysate 20 µg Lane 2: Raji whole cell lysate 20 µg Lane 3: Jurkat whole cell lysate 20 µg Negative control: MCF7 whole cell lysate Low expression control: Raji whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 180~240 kDa Observed MW: 240~260 kDa Exposure time: 30s

IHC shows positive staining in paraffin-embedded human tonsil.Anti-CD45 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human spleen.Anti-CD45 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon.Anti-CD45 antibody was used at 1/250 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon.Anti-CD45 antibody was used at 1/250 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human cervix cancer.Anti-CD45 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human diffuse large B-cell lymphoma.Anti-CD45 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

Negative control: IHC shows negative staining in paraffin-embeddedhuman skeletal muscle.Anti-CD45 antibody was used at 1/1000 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
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