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worldwide@absin.cn
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Rabbit anti-CD11b Polyclonal Antibody
Integrin alpha-M
Reactivity：
Human
Application：
WB, IP, IHC-P
Host：
Rabbit
Clonality：
Monoclonal antibody
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Experimental result diagram
WB result of CD11b Rabbit mAb Primary antibody:CD11b Rabbit mAb at 1/1000 dilution Lane 1: Jurkat whole cell lysate 20 µg Lane 2: U937 whole cell lysate 20 µg Lane 3: TF-1 whole cell lysate 20 µg Lane 4: THP-1 whole cell lysate 20 µg Negative control: Jurkat whole cell lysate Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 170 kDa Observed MW: 180 kDa Exposure time: Lane 1、lane 2 and lane 4: 180s Lane 3: 20s
IHC shows positive staining in paraffin-embedded human tonsil. Anti-CD11b antibody was used at 1/500 dilution,Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human spleen. Anti-CD11b antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human stomach. Anti-CD11b antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung. Anti-CD11b antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human cervix cancer. Anti-CD11b antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
IHC shows positive staining in paraffin-embedded human lung squamous cancer. Anti-CD11b antibody was used at 1/500 dilution, Secondary antibody: #abs20040. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.
CD11b Rabbit mAb at 1/25 dilution (2µg) immunoprecipitating CD11b in 0.4mg TF-1 whole cell lysate. Western blot was performed on the immunoprecipitate using CD11b Rabbit mAb at 1/1000 dilution. Secondary antibody (HRP) for IP was used at 1/400 dilution. Lane 1: TF-1 whole cell lysate 10µg (input) Lane 2 (+): CD11b Rabbit mAb IP in TF-1 whole cell lysate Lane 3 (-): Rabbit monoclonal IgG IP in TF-1 whole cell lysate Predicted MW: 170 kDa Observed MW: 180 kDa Exposure time: 10s
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