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      abs125057

      Rabbit anti-Glutamine Synθse Polyclonal Antibody

      GLNA; GLNS; GLUL; Gl Syn; glutamate-ammonia ligase; glutamine synthetase; GS; PIG 43; PIG 59; PIG43; PIG59; Proliferation inducing protein 43
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      Reactivity:
      Human
      Application:
      WB, IHC-P, IHC-F, IF, ELISA
      Host:
      Rabbit
      Clonality:
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Sample: Raji Cell (Human) Lysate at 40 ug
      Primary: Anti- Glutamine synthetase (abs125057) at 1/300 dilution
      Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
      Predicted band size: 42 kD
      Observed band size: 42 kD

      Tissue/cell: mouse brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
      Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
      Incubation: Anti-Glutamine synthetase Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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