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      abs120021

      Rabbit anti-Collagen III Polyclonal Antibody

      COL 3A1; COL3A1; Collagen alpha 1(III) chain; Collagen III alpha 1 chain precursor; Collagen III alpha 1 polypeptide; Collagen type III alpha 1 (Ehlers Danlos syndrome type IV autosomal dominant); Collagen type III alpha 1; Collagen type III alpha; EDS4A; Ehlers Danlos syndrome type IV; autosomal dominant; Fetal collagen; Type III collagen; CO3A1_HUMAN; Collagen alpha-1(III) chain; type III preprocollagen alpha 1 chain,Collagen III
      Product ImageProduct Image
      Reactivity:
      Human, Dog, Rabbit
      Application:
      WB, IHC-F, IHC-P, IF
      Host:
      Rabbit
      Clonality:
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Sample: Lane 1: Human HeLa cell lysates Lane 2: Human MCF-7 cell lysates Primary: Anti-Collagen III at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 117 kDa Observed band size: 130 kDa

      Tissue/cell: dog bladder tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum) at 37℃ for 20 min; Incubation: Anti-Collagen III Polyclonal Antibody, Unconjugated 1:800, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

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