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      abs132005

      Rabbit anti-Cleaved-caspase3 Polyclonal Antibody

      A830040C14Rik; Apopain; CASP-3; CASP3; CASP3_HUMAN; Casp3a; Caspase 3; apoptosis-related cysteine peptidase; apoptosis-related cysteine protease; apoptosis-related cysteine protease a; Caspase-3 subunit p12; CC3; CPP-32; CPP32; CPP32B; Cysteine protease CPP32; EC 3.4.22.56; LICE; mldy; OTTHUMP00000165052; OTTHUMP00000165053; OTTHUMP00000165054; PARP cleavage protease; Procaspase3; protein Yama; SCA 1; SCA-1; SREBP cleavage activity 1; Yama,Cleaved CASP3
      Product ImageProduct Image
      Reactivity：
      Human, Mouse, Rat, Bovine
      Application：
      WB, IHC, IF, ICC
      Host：
      Rabbit
      Clonality：
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Western blot analysis of extracts from various samples, using Cleaved-Caspase 3 (Asp175), p17 Ab. Lane 1: Rat liver, blocked with antigen-specific peptides. Lane 2: Rat liver. Lane 3: Rat spleen.

      abs132005 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(1:200) and mouse anti-beta tubulin Ab(1:200) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).

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