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      abs119676

      Rabbit anti-Caspase 3 Polyclonal Antibody

      Caspase-3 subunit p17; APOPAIN; CASP3; Caspase 3 apoptosis related cysteine protease; Caspase3; CPP32; CPP32B; Cysteine protease CPP32; Human cysteine protease CPP32 isoform alpha mRNA complete cds; PARP cleavage protease; SCA 1; SCA1; SREBP cleavage activity 1; Yama; CASP3_HUMAN; Caspase-3; CASP-3; Apopain; Protein Yama; SCA-1,CASP3
      Product ImageProduct Image
      Reactivity：
      Human, Mouse, Rat
      Application：
      WB, ELISA
      Host：
      Rabbit
      Clonality：
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Sample: Lane 1: Spleen (Mouse) Lysate at 40 ug Lane 2: Lung (Mouse) Lysate at 40 ug Lane 3: Lymph node (Mouse) Lysate at 40 ug Lane 4: Cerebrum (Mouse) Lysate at 40 ug Lane 5: NIH/3T3 (Mouse) Cell Lysate at 30 ug Lane 6: Spleen (Rat) Lysate at 40 ug Lane 7: Lung (Rat) Lysate at 40 ug Lane 8: Lymph node (Rat) Lysate at 40 ug Lane 9: Cerebrum (Rat) Lysate at 40 ug Lane 10: Hela (Human) Cell Lysate at 30 ug Primary: Anti-Caspase-3 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 35 kD Observed band size: 37 kD

      Tissue/cell: NIH/3T3 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (Caspase-3) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a FITC conjugated Goat Anti-Rabbit IgG antibody at 37°C for 90 minutes, DAPI (blue) was used to stain the cell nuclei.

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