Rabbit anti- BDNF Monoclonal Antibody

25 ug total protein per lane of various lysates (see on figure) probed with BDNF monoclonal antibody, unconjugated at 1:1000 dilution and 4°C overnight incubation. Followed by conjugated secondary antibody incubation at r.t. for 60 min.

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S

Paraformaldehyde-fixed, paraffin embedded Rat Cerebrum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP

Paraformaldehyde-fixed, paraffin embedded Rat Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the S

Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the

Paraformaldehyde-fixed, paraffin embedded Human Brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; Antibody incubation with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation to the SP K

Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugate

Paraformaldehyde-fixed, paraffin embedded Rat Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by conjugated

Paraformaldehyde-fixed, paraffin embedded Human left parietal lobe; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C. Followed by co

Paraformaldehyde-fixed, paraffin embedded Mouse Spinal Cord; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugatio

Paraformaldehyde-fixed, paraffin embedded Mouse Cerebellum; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min; The section was incubated with BDNF Monoclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by conjugation

The U-87 MG (H) cells were fixed with 4% PFA (10 min at r.t.) and then permeabilized with 90% ice-cold methanol for 20 min at -20℃,the cells then were incubated in 5%BSA to block non-specific protein-protein interactions (30 min at r.t.), followed by sec