Rabbit anti-Angiotensin II Type 1A Receptor Polyclonal Antibody

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

Sample: Lane 1: Liver (Mouse) Lysate at 40 ug Lane 2: Heart (Mouse) Lysate at 40 ug Lane 3: Placenta (Mouse) Lysate at 40 ug Lane 4: Kidney (Mouse) Lysate at 40 ug Lane 5: Embryo (Mouse) Lysate at 40 ug Lane 6: Cerebrum (Mouse) Lysate at 40 ug Lane 7: NIH/3T3 (Mouse) cell Lysate at 30 ug Lane 8: Liver (Rat) Lysate at 40 ug Lane 9: Heart (Rat) Lysate at 40 ug Lane 10: Kidney (Rat) Lysate at 40 ug Lane 11: Cerebrum (Rat) Lysate at 40 ug Lane 12: Embryo (Rat) Lysate at 40 ug Primary: Anti-Angiotensin II type 1A receptor at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 43 kD Observed band size: 46 kD

Paraformaldehyde-fixed, paraffin embedded (rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (Angiotensin II type 1A receptor) Polyclonal Antibody, Unconjugated at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.