Rabbit anti-AIF1 Polyclonal Antibody

AIF 1; AIF1; AIF1 protein; IBa1; iba-1; IBa 1; allograft inflammatory factor 1; Allograft inflammatory factor 1 splice variant G; balloon angioplasty responsive transcription; BART 1; G1; G1 putative splice variant of allograft inflamatory factor 1; IBA 1; IBA1; interferon gamma responsive transcript; Ionized calcium binding adapter molecule 1; ionized calcium-binding adapter molecule; IRT 1; IRT1; Microglia response factor; MRF1; Protein G1

Reactivity：

Human, Mouse, Rat, Pig

Application：

WB, IHC-P, IHC-F, FCM, ELISA

Host：

Rabbit

Clonality：

Polyclonal Antibody

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Tips : This product is for research use only. Not for use in diagnostic prodcedures.

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Experimental result diagram

Sample: Brain(Mouse) lysate at 30 ug ;
Primary:Anti-AIF1/Iba1 (abs122767) at 1:300 dilution;
Secondary: HRP conjugated Goat-Anti-rabbit IgG at 1: 5000 dilution;
Predicted band size: 16 kD
Observed band size: 16 kD

Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-AIF1/IBa-1 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining

Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (AIF1 Iba1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer at 37°C for 30min; Antibody incubation with (AIF1 Iba1) Polyclonal Antibody, Unconjugated secondary primary antibody at 1:400 overnight at 4°C, followed by a conjugated Goat Anti-Rabbit IgG antibody for 90 minutes, and DAPI for nuclei staining.

Tissue/cell: rat brain tissue;4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer, Boiling bathing for 15min; Blocking buffer at 37℃ for 20 min;
Incubation: Anti-AIF1/Iba1 Polyclonal Antibody, Unconjugated secondary primary antibody 1:200, overnight at 4°C; The secondary antibody was Goat Anti-Rabbit IgG, Cy3 conjugatedused at 1:200 dilution for 40 minutes at 37°C. DAPI(5ug/ml,blue) was used to stain the cell nuclei