Rabbit anti-β-Actin Monoclonal Antibody

ACTB

Reactivity：

Human, Mouse, Rat

Application：

WB, IHC, ICC, FCM (Intra)

Host：

Rabbit

Clonality：

Monoclonal antibody

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Tips : This product is for research use only. Not for use in diagnostic prodcedures.

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Experimental result diagram

WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: Hela whole cell lysate 20 µg Lane 2: Jurkat whole cell lysate 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 43 kDa Observed MW: 43 kDa Exposure time: 15s

WB result of β-actin Rabbit mAb Primary antibody: β-actin Rabbit mAb at 1/1000 dilution Lane 1: rat brain lysate 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 43 kDa Observed MW: 43 kDa Exposure time: 15s

IHC shows positive staining in paraffin-embedded smooth msucle of human skeletal muscle. Anti-β-actin antibody was used at 1/2000 dilution, Secondary antibody: #abs20040 Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon. Anti-β-actin antibody was used at 1/2000 dilution, Secondary antibody: #abs20040 Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded human colon cancer. Anti-β-actin antibody was used at 1/2000 dilution, Secondary antibody: #abs20040 Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse liver. Anti-β-actin antibody was used at 1/2000 dilution, Secondary antibody: #abs20040 Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

ICC shows cytoplasm staining in HeLa cells. Anti-β-actin antibody was used at 1/500 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution.The cells were fixed with 100% Methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.

ICC shows cytoplasm staining in NIH3T3 cells. Anti-β-actin antibody was used at 1/500 dilution and incubated overnight at 4°C. Secondary antibody: #abs20025 at 1/1000 dilution.The cells were fixed with 100% Methanol and permeabilized with 0.1% PBS-Triton X-100. Nuclei were countersained with DAPI.

Flow cytometric analysis of HeLa cells labelling β-actin antibody at 1/500 (0.1ug) dilution/ (red) compared with a Rabbit monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Secondary antibody：#abs20025.