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Indirect ELISA antibody dose-response curve using Human Tau non-phospho and Human Tau phospho T181 peptides. Peptide concentration was 500 ng/mL. Tau (phospho T181) Recombinant Rabbit mAb was added at 0-100 ng/mL. Samples were incubated with Peroxidase-AffiniPure Goat Anti-Rabbit IgG (H+L) secondary antibody at 1/10000 dilution.
WB result of Tau(phospho T181) Rabbit mAb Primary antibody: Tau(phospho T181) Rabbit mAb at 1/1000 dilution Lane 1: old mouse brain lysate 20 µg Lane 2: old mouse brain lysate(phosphatase treated) 20 µg Secondary antibody: #abs20040 at 1/10000 dilution Predicted MW: 50~70 kDa Observed MW: 63 kDa Exposure time: 60s
Dot blot result of Tau(phospho T181) Rabbit mAb Lane 1: Tau phospho T181 peptide Lane 2: Tau non-phospho T181 peptide Primary antibody: Tau(phospho T181) Rabbit mAb at 1/1000 dilution Secondary antibody: #abs20040 at 1/10000 dilution Exposure time: 20s
