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      abs115033

      Rabbit anti-PROM1 Polyclonal Antibody

      RP41; AC133; CD133; MCDR2; STGD4; CORD12; PROML1; MSTP061
      Product ImageProduct ImageProduct ImageProduct Image
      Reactivity:
      Human, Mouse, Rat
      Application:
      WB, IF/ICC, ELISA
      Host:
      Rabbit
      Clonality:
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Western blot analysis of lysates from HCT116 cells, using CD133 Rabbit pAb at 1:1000 dilution.
      Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
      Lysates/proteins: 25μg per lane.
      Blocking buffer: 3% nonfat dry milk in TBST.
      Detection: ECL Basic Kit .
      Exposure time: 30s.

      Western blot analysis of various lysates using CD133 Rabbit pAb at 1:1000 dilution.
      Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
      Lysates/proteins: 25μg per lane.
      Blocking buffer: 3% nonfat dry milk in TBST.
      Detection: ECL Basic Kit .
      Exposure time: 90s.

      Immunofluorescence analysis of NIH/3T3 cells using CD133 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

      Immunofluorescence analysis of U-2 OS cells using CD133 Rabbit pAb at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

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