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      abs520005 (12 Citations)

      Mouse IL-10 ELISA Kit

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      Detection Principle:

      This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti mouse il-10antibody was pre coated on a high affinity microplate. The standard, sample to be tested and biotinylated detection antibody are added into the wells of the enzyme plate. After incubation, the il-10present in the sample combines with the solid-phase antibody and detection antibody to form an immune complex. After washing to remove unbound material, horseradish peroxidase labeled streptavidin (streptavidin HRP) was added. After washing, the chromogenic substrate was added to avoid light for color development. Stop the reaction by adding stop solution, and determine the absorbance value at 450 nmwavelength (reference correction wavelength 540nmor 570nm)

      Detection Type: double antibody sandwich method

      Form: pre coated 96orifice plate

      Detection Sample Type: cell supernatant, serum, Plasma

      Loading amount: 100ul

      Kit Components:

      Pre coated 96well plates, standards, anti mouse il-10detection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrpA copy of sealing film and instructions.

      Sensitivity: 1.8 pg/ml

      Detection Range: 31.2 - 2000 pg/ml

      Recovery Range: 95-103%

      Storage Method: 2-8℃

      Background:

      Mouse interleukin -10 (IL-10) also known as cytokine synthesis inhibitor (CSIF). It is IL-10 & Alpha-a member of the helical cytokine family, which also includes il-19, il-12, il-22, IL-24 , and il-26/ak155. Many activated hematopoietic stem cells, hepatic stellate cells, keratinocytes, and placental cytotrophoblasts secrete il-10. Human IL-10 is active in mouse cells, but mouse IL-10 does not act on human cells. The amino acid homology of mature mouse IL-10 and rat IL-10 is 85%, and that of cattle, dogs, horses, cats, humans, sheep, and pigs is 70~77%. Mature mouse IL-10 is a noncovalently bound homodimer with a molecular weight of 36 kDa containing two intrachain disulfide bonds.

      IL-10 by the II type cytokine receptor IL-10 r& Alphaand IL-10 & amp; Betathe heteromeric receptor complex that is composed of exerts its biological activityIL-10 r& Alphais a 110 kDa transmembrane glycoprotein expressed in lymphocytes, natural killer cells, macrophages, monocytes, astrocytes, intestinal epithelial cells, cytotrophoblasts, and activated hepatic stellate cells, while 75 kDa il-10r& Beta

      IL-10 is involved in immune regulation of inhibitory and activating effectsIL-10 as an anti-inflammatory cytokine, promotes the formation of M2 macrophages by inhibiting the activation and expansion of Th1 cells and Th17 cells. Immunosuppression regulates the expression of T cells and B cells, which play an important role in Treg cell proliferation (19) . In the tumor microenvironment, IL-10 inhibition regulates the proliferation of T cells and myeloid derived suppressor cellsIL-10 induces the accumulation of intratumoral material and activates cd8+ T cellsIL-10 plays an important protective role in limiting tissue damage in joint inflammation and promoting muscle regrowth after injury, but also contributes to persistent viral infection. IL-10 is elevated in Sjogren's syndrome (saliva) and primary central nervous system lymphoma.

      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
      Dear customer, if you have any questions or want to know about our products, you can click the "I want to ask" button to fill in your questions. Unsuccessful submission? Please contact worldwide@absin.cn.
      Ask questions
      Therapeutic potential of isoallolithocholic acid in methicillin-resistant Staphylococcus Aureus peritoneal infection

      Ying Lu,Jun Du,Shicheng Peng,Ying Wang,Yongtao Xiao

      J Antibiot (Tokyo). 2024 Dec 17 .

      Influencing factors:2.100

      Yishen Huazhuo decoction regulates microglial polarization to reduce Alzheimer's disease-related neuroinflammation through TREM2

      Kai Wang, Shujie Zan, Jiachun Xu, Weiming Sun, Caixia Li, Wei Zhang, Daoyan Ni, Ruzhen Cheng, Lin Li, Zhen Yu, Linlin Zhang, Shuang Liu, Yuanwu Cui, Yulian Zhang

      Heliyon. 2024 Aug 6 .

      Influencing factors:3.400

      Matrix stiffness regulates macrophage polarisation via the Piezo1‐YAP signalling axis

      Feng Mei, Yaru Guo, Yu Wang, Yingying Zhou, Boon Chin Heng, Mengru Xie, Xiaofei Huang, Shihan Zhang, Shuai Ding, Fangyong Liu, Xuliang Deng, Lili Chen, Cheng Yang

      Cell Prolif. 2024 Mar 31 .

      Influencing factors:5.900

      Ferritin-mediated neutrophil extracellular traps formation and cytokine storm via macrophage scavenger receptor in sepsis-associated lung injury

      Hao Zhang, Dan Wu, Yanghanzhao Wang, Yuxin Shi, Yuwen Shao, Fu Zeng, Charles B. Spencer, Lilibeth Ortoga, Dehua Wu, Changhong Miao

      Cell communication and signaling. 2024 Feb 2 .

      Influencing factors:8.400

      Roflumilast attenuates neuroinflammation post retinal ischemia/reperfusion injury by regulating microglia phenotype via the Nrf2/STING/NF-κB pathway

      Yuyan Guo, Chunlian Ou, Naiyuan Zhang, Qiong Liu, Ke Xiong, Jian Yu, Hao Cheng, Linjiang Chen, Ming Ma, Jing Xu, Jing Wu

      INTERNATIONAL IMMUNOPHARMACOLOGY. 2023 Sep 24 .

      Influencing factors:5.6

      Marsdenia tenacissima enhances immune response of tumor infiltrating T lymphocytes to colorectal cancer

      Ben Yi, Shuai Zhang, Suying Yan, Yanfei Liu, Zhiqiang Feng, Tianhao Chu, Jun Liu, Wei Wang, Jun Xue, Chunze Zhang, Yijia Wang

      Frontiers in Immunology. 2023 Aug 15 .

      Influencing factors:7.3

      MeMFS对Aβ25-35诱导海马神经元—小胶质细胞共培养体系中细胞因子分泌的影响

      张小菊

      遵义医科大学. 2023 Jun 30 .

      过表达IL-37a的MSC对LPS诱导的ARDS的免疫调控研究

      李艳群;李锦灵;王丽莎;李治寰;谭凤鸣

      西南民族大学学报(自然科学版). 2023 Jan 25 .

      Severe Inflammation Caused by Coinfection of PCV2 and Glaesserella parasuis Is Associated with Pyroptosis via Noncanonical Inflammasome Pathway

      Jiahui An, Chao Zhang, Jinshuang Cai, Yufeng Li

      Cellular microbiology. 2022 Nov 16 .

      Influencing factors:2.600

      Tailoring Aggregation Extent of Photosensitizers to Boost Phototherapy Potency for Eliciting Systemic Antitumor Immunity

      Hao Zhao, Jiabao Xu, Chan Feng, Jiayu Ren, Lin Bao, Yanbing Zhao, Wei Tao, Yuliang Zhao, Xiangliang Yang

      Advanced Materials. 2021 Nov 15 .

      Influencing factors:30.849

      阿魏酸对RAW264.7源性泡沫细胞脂代谢及炎症反应的影响

      赵立凤;罗瑞熙;许滔;俞琦;李帅帅

      贵州中医药大学学报. 2021 Mar 30 .

      黄连解毒汤含药血清激活PPARγ诱导RAW264.7源性泡沫细胞向M2表型极化

      盛蒙许滔; 于红红; 赵立凤; 俞琦; 田维毅

      中国免疫学杂志. 2020 Feb 12 ; 3:-

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