Mouse IL-1α ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse il-1α Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The il-1&alpha in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color reaction and il-1&alpha in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, biotin labeled il-1α One copy of detection antibody, standard dilution, detection buffer, sa-hrp, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 1.68 pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 71-106%

Storage method: 2-8 ℃

Standard Curve:

Background:

Interleukin 1 (IL-1)It is a 17kDa extracellular polypeptide, which is divided into il-1α And il-1β Both proteins. Il-1α Is a pleiotropic cytokine encoded by the IL1A gene in humans. Il-1α It is mainly produced by activated macrophages, neutrophils, epithelial cells and endothelial cells. It has metabolic, physiological and hematopoietic activities and plays a central role in regulating the immune response.

IL-1α It can interact with HAX1 and NDN, but the synergy with TNF is the most clinically relevant. Il-1α And TNF are both acute phase cytokines with Pro febrile and inflammatory effects.

People are currently evaluating il-1α Whether it can be used as a potential tumor treatment marker in clinical trials. Blocking il-1α Activity has potential therapeutic effects on skin diseases, such as acne.