Mouse CCL3/MIP-1α ELISA Kit

Detection principle:

In this experiment, a double antibody sandwich was used ELISA Law. anti-mouse MIP-1α Monoclonal antibody coating on microplates, samples and standards MIP-1α It will bind to the antibody immobilized on the plate, and free components will be washed away; Anti-mouse labeled with horseradish peroxidase MIP-1α Multiple antibodies, and those bound to microplates MIP-1α By binding to form an immune complex, free components are washed away; When the substrate solution (developer) is added, the color of the solution gradually turns blue, when the stop solution is added, it turns yellow and stops changing. Absorbance was determined with a microplate reader.

Test Type: double antibody sandwich method

Format: pre-wrapped 96 orifice plate

Test Sample Type: Cell supernatant, serum, plasma

Sample Load: 100ul

Kit Components:

pre-wrapped 96 Well plates, standards, anti-mouse MIP-1α Detection antibody, dilution buffer, chromogenic solution ( A 、 B ), washing solution, termination solution, SA-HRP One copy of the sealing film and the instruction manual.

Sensitivity: 1.5 pg/mL

Detection range: 7.81 - 500 pg/mL

Recovery range: 85.0-111.9%

Storage method: 2-8℃

Standard graph

Background:

MIP-1 alpha (Macrophage inflammatory protein 1 alpha ) is CC or beta Members of the chemokine subfamily, originally from LPS Purified in conditioned medium of stimulated murine macrophage lines. MIP-1α For a variety of cells (including monocytes, T cells, B Cells and eosinophils) act as chemotaxis.
Two humans MIP-1α Genes are replicated / Mutations occur. They code MIP-1α isoform CCL3 / LD78a and CCL3L1 / LD78b They have 94 % amino acid sequence homology. people CCL3 / LD78a It is a single-copy gene, while humans CCL3L1 / LD78b Gene copy numbers vary across populations. people CCL3L1 / LD78b Binds and passes through chemokine receptors CCR1 ， CCR5 Send out a signal. with CCL3 / LD78a In comparison, CCL3L1 / LD78b Right CCR5 Has higher binding affinity, CCR5 Also HIV-1 Entry co-receptors. CCL3L1 The copy number is HIV-1 One of several genetic determinants of susceptibility.