Human sTNF RI/TNFRSF1A ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human TNF RI capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The TNF RI in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of TNF RI in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, TNF RI detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.08pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 98-109%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Tumor necrosis factor receptor 1 (TNFRI), also known as tumor necrosis factor receptor superfamily member 1a (TNFRSF1A)And CD120a, a member of the tumor necrosis factor receptor superfamily. It can activate the transcription factor NF - & kappa; B. Mediates apoptosis and regulates inflammation. Mutations in the TNFRI gene are associated with an increased risk of multiple sclerosis. The serum levels of TNFRI in patients with schizophrenia and bipolar disorder are elevated, and high levels of TNFRI are associated with more severe psychiatric symptoms