Human RAGE ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human rage capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for a 2-hour incubation process. Rage in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of rage in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, rage detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.75pg/ml

Detection Tange: 15.63-1000 pg/ml

Recovery Range: 89-108%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Receptor for advanced glycation end products (RAGE)Is a 35kDa immunoglobulin superfamily transmembrane receptor, whose name comes from its ability to bind advanced glycation end products. Given its inflammatory function in innate immunity and its ability to recognize a class of ligands through common motifs, rage is generally considered a class of recognition receptors. Rage is associated with some chronic diseases, such as atherosclerosis, peripheral vascular disease, myocardial infarction, congestive heart failure, diabetes, Alzheimer's disease, etc. Compared with other tissues, rage has the highest expression in the lung, especially in type I alveolar epithelial cells, but not in idiopathic pulmonary fibrosis (IPF), suggesting that the expression and regulation of rage in the lung system is different from that in the cardiovascular system. Blocking / knocking down rage will lead to impaired cell adhesion and increased cell proliferation and migration.