Human / Porcine Insulin ELISA Kit

Testing Principle

This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti- Human / Porcine Insulin antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the Human / Porcine Insulin present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

Detection type: sandwich enzyme immunoassay

Form: pre-coated 96-well plate

Sample type: Cell culture supernatant, Serum, Plasma

Sample quantity: 100ul

Kit composition:

96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

Sensitivity:   2.15 pmol / mL

Detection range:   15.6 - 1000 pmol/mL

Recovery rate:    85.0-108.9%

Storage: 2-8 ° C

The standard curve:

Background: Insulin

Insulin and IGF are members of the insulin molecule family. IGF-I and II are structurally homologous to proinsulin and can be thought of as an exaggerated inverted G shape. The G part of the chain is inchain disulfide bonded. Single-stranded proinsulin peptide is composed of 30 amino acid residues of B chain (aa 2554), C peptide (aa 5589) and 21 amino acid residues of A chain (aa 90110).The removal of C peptide by proteolysis results in the formation of mature insulin, which is the heterodimers formed by disulfide bonds between A and B chains. Circulating C peptide levels are elevated in hyperinsulinemia, obesity and type 2 diabetes.

Technical hints: For research use only, not for in vitro diagnosis.