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      abs510020 (1 Citations)

      Human IL-22 ELISA Kit

      Cytokine Zcyto18; IL-10-related T-cell-derived inducible factor; IL-21; IL22; IL-22; IL-22IL21; IL-D110; IL-TIF; ILTIFIL-10-related T-cell-derived-inducible factor; IL-TIFMGC79382; interleukin 21; interleukin 22; interleukin-22; MGC79384; TIFa; TIFIL-23; zcyto18
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      Testing Principle

      This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).

       

      Detection type: sandwich enzyme immunoassay

      Form: pre-coated 96-well plate

      Sample type: Cell culture supernatant, Serum, Plasma

      Sample quantity: 100ul

      Kit composition:

      96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification

      Sensitivity:  1.8 pg / mL

      Detection range:   31.3 - 2000 pg/mL

      Recovery rate:  82-110%

      Storage: 2-8 ° C

      The standard curve:

      Background: IL-22

      Il-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.

      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
      Dear customer, if you have any questions or want to know about our products, you can click the "I want to ask" button to fill in your questions. Unsuccessful submission? Please contact worldwide@absin.cn.
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      Inflammatory endotypes of adenoidal hypertrophy based on a cluster analysis of biomarkers

      Hong-li Hua, Yu-qin Deng, Huan Huang, Yu-chen Tang, Ji-bo Han, Fen Li, Yan Wang, Ze-zhang Tao

      INTERNATIONAL IMMUNOPHARMACOLOGY. 2023 Dec 11 .

      Influencing factors:5.600

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