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Human IL-22 ELISA Kit


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Testing Principle
This kit uses quantitative sandwich enzyme immunoassay technique. A specific anti-human IL-22 antibody is pre-coated on a high affinity plate. Standards, test samples, and biotinylated detection antibodies are added to the wells of the enzyme-labeled plate. After incubation, the IL-22 present in the samples combines with the solid-phase antibodies and the detection antibodies to form immune complexes. After washing to remove unbound material, horseradish peroxidase-labeled streptavidin (HRP) was added. After washing, a chromogenic substrate was added, and the color was developed in the dark. The reaction was stopped by adding a stop solution, and the absorbance was measured at a wavelength of 450 nm (reference correction wavelength of 540 nm or 570 nm).
Detection type: sandwich enzyme immunoassay
Form: pre-coated 96-well plate
Sample type: Cell culture supernatant, Serum, Plasma
Sample quantity: 100ul
Kit composition:
96 well polysyrene microplate coated with capture antibody, standard, detection antibody, 10×reagent diluent, Color reagent (A and B), 25×wash buffer, Stop solution, ELISA plate sealers, specification
Sensitivity: 1.8 pg / mL
Detection range: 31.3 - 2000 pg/mL
Recovery rate: 82-110%
Storage: 2-8 ° C
The standard curve:
Background: IL-22
Il-22 (interleukin-22) is a cytokine that induces ros, IL-6, IL-10, TNF -α, and neutrophil infiltration.It also supports the integrity of the epithelial barrier and induces epithelial cell proliferation during wound healing.The IL-22 signal is transmitted through a receptor complex consisting of IL-22R and IL-10R.Il-10 R beta is a common component of IL-10, IL-26, IL-28 and IL-29 receptor complexes.Il-22 binds to IL-22BP and blocks the interaction between IL-22 and IL-22R.