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      abs120942

      Rabbit anti-HB9 Polyclonal Antibody

      HB9/HLXB9; HB 9; HB9; HLXB 9; HLXB9; Homeo box HB9; Homeobox HB9; Homeobox protein HB9; HOXHB9; MNX1; MNX1_HUMAN; Motor neuron and pancreas homeobox protein 1; Sacral agenesis autosomal dominant (Currarino triad); SCRA 1; SCRA1,HB9
      Product ImageProduct ImageProduct ImageProduct ImageProduct ImageProduct Image
      Reactivity：
      Human, Mouse, Rat
      Application：
      WB, IF/ICC, FCM
      Host：
      Rabbit
      Clonality：
      Polyclonal Antibody
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Experimental result diagram

      Positive control: RSC96 Isotype Control Antibody: Rabbit IgG ; Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 1:100 in 1 X PBS containing 0.5% BSA ; Primary Antibody Dilution: 6μg in 100 μL1X PBS containing 0.5% BSA.

      Sample: Lane 1: Pancreas (Mouse) Lysate at 40 ug Lane 2: Pancreas (Rat) Lysate at 40 ug Primary: Anti-MNX1/HLXB9 at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD

      Black line : Positive blank control RSC96); Negative blank control (HL60） Green line : Primary Antibody (Rabbit Anti- HLXB9 antibody ) Orange line：Isotype Control Antibody (Rabbit IgG) . Blue line : Secondary Antibody (Goat anti-rabbit IgG-AF488)

      Blank control: K562. Primary Antibody (green line): Rabbit Anti-MNX1 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells

      Blank control: K562. Primary Antibody (green line): Rabbit Anti-MNX1 antibody Dilution: 2μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITC Dilution: 0.5μg /test. Protocol The cells

      K562 cell; 4% Paraformaldehyde-fixed; Triton X-100 at room temperature for 20 min; Blocking buffer (normal goat serum) at 37°C for 20 min; Antibody incubation with (MNX1) polyclonal Antibody, Unconjugated 1:100, 90 minutes at 37°C; followed by a conjugat

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