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  • Goat serum
  • Normal goat serum for closure; Goat serum
  • SKU: abs933
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    Product Details FAQ Citations(22) Video Pictures Documents
    Tips:This product is for research use only. Not for use in diagnostic prodcedures.
    Description
    Description

    Normal serum

    Application
    1. Animal immunity and preparation of serum IgG.
    2. Immunohistochemical blocking, ELISA and other immunoassay blocking and the use of protective agents.
    Usage
    Immunohistochemistry: Block with 5-10% normal goat serum (1:10-1:20-fold dilution) and incubate at room temperature for 5-10 minutes. Remove serum, do not wash, add appropriate proportion of diluted primary antibody or primary antibody working solution, incubate at 37℃ or room temperature at 4℃ overnight. Cell smear: Sealed with 5-10% normal goat serum (1:10-1:20 fold dilution), diluted with PBS (0.01mol/L pH7.4), a small amount of drip: pass the cell smear. ELISA: 1:100 fold diluted with PBS (0.01mol/L pH7.4) and the sealing time was 37℃ an hour.
    Overview
    Storage Temp.
    Store at 2-4℃ for 2-3 weeks; -20℃ for 3 years; Avoid repeated freeze-thaw cycles.
    Properties
    Synonym
    Normal goat serum for closure; Goat serum
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