Description | |
Description | Normal serum |
Application | 1. Animal immunity and preparation of serum IgG. 2. Immunohistochemical blocking, ELISA and other immunoassay blocking and the use of protective agents. |
Usage | Immunohistochemistry: Block with 5-10% normal goat serum (1:10-1:20-fold dilution) and incubate at room temperature for 5-10 minutes. Remove serum, do not wash, add appropriate proportion of diluted primary antibody or primary antibody working solution, incubate at 37℃ or room temperature at 4℃ overnight. Cell smear: Sealed with 5-10% normal goat serum (1:10-1:20 fold dilution), diluted with PBS (0.01mol/L pH7.4), a small amount of drip: pass the cell smear. ELISA: 1:100 fold diluted with PBS (0.01mol/L pH7.4) and the sealing time was 37℃ an hour. |
Overview | |
Storage Temp. | Store at 2-4℃ for 2-3 weeks; -20℃ for 3 years; Avoid repeated freeze-thaw cycles. |
Properties | |
Synonym | Normal goat serum for closure; Goat serum |
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