Mouse GM-CSF ELISA Kit

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. The anti mouse GM-CSF monoclonal antibody is coated on the microplate, the GM-CSF in the sample and standard will bind with the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse GM-CSF polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Detection Type: double antibody sandwich method

Form: pre coated 96orifice plate

Detection Sample Type: cell supernatant, Serum

Loading Amount: 100ul

Kit Components:

Pre coated 96well plates, standards, anti mouse  gm-csfdetection antibody, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: to be improved

Detection Range: 7.81-500 pg/ml

Recovery Range: 84-110%

Preservation Method: 2-8℃

Standard Curve

Background:

GM-CSF (granulocyte macrophage colony stimulating factor)Induce the development of monocytes, neutrophils, eosinophils, myeloid dendritic cells and dermal dendritic cells. It also acts as a chemoattractant for neutrophils and dendritic cells. GM-CSF promotes Th1 and Th17 cell-mediated autoimmune inflammation and inflammatory activation of dendritic cells, microglia, alveolar macrophages, and eosinophils. In addition, it synergizes with G-CSF to promote the proliferation and invasion of tumor cells. GM-CSF signaling is mediated through the receptor complex, which is composed of GM-CSF R & amp; Alpha; And beta C subunit, syndecan-2 as a potential co receptor. The beta C subunit is shared by the receptor complex of IL-3 and IL-5.