Rabbit anti-GAPDH Polyclonal Antibody

WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/10000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: THP-1 whole cell lysate 20 ug Lane 3: MCF7 whole cell lysate 20 ug Lane 4: NIH/3T3 whole cell lysate 20 ug Lane 5: mouse spleen lysate 20 ug Lane 6: C6 whole cell lysate 20 ug Lane 7: rat spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s

WB result of GAPDH Rabbit mAb Primary antibody: GAPDH Rabbit mAb at 1/50000 dilution Lane 1: HeLa whole cell lysate 20 ug Lane 2: THP-1 whole cell lysate 20 ug Lane 3: MCF7 whole cell lysate 20 ug Lane 4: NIH/3T3 whole cell lysate 20 ug Lane 5: mouse spleen lysate 20 ug Lane 6: C6 whole cell lysate 20 ug Lane 7: rat spleen lysate 20 ug Secondary antibody: Goat Anti-Rabbit IgG, (H+L), HRP conjugated at 1/10000 dilution Predicted MW: 37 kDa Observed MW: 37 kDa Exposure time: 180 s

IHC shows positive staining in paraffin-embedded human spleen. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded mouse kidney. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.

IHC shows positive staining in paraffin-embedded rat testis. Anti-GAPDH antibody was used at 1/5000 dilution, followed by a HRP Polymer for Goat Anti-Rat IgG (H+L). Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris/EDTA buffer pH9.0 was performed before commencing with IHC staining protocol.