Description | |
Description | This product has an excellent role in promoting cell growth and product stability, after three times 0.1 micron filtration, with sterile, no mycoplasma, no specific antibody, low endotoxin, good promoting cell growth and other characteristics. |
Appearance | Light yellow clarification of slightly viscous liquid |
Form | Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol). |
Technical Index | Bovine immunoglobulin content: < 500mg/L; |
Applications | Suitable for specific antibody requirements of higher products, more expensive cells, cell culture and closure. |
Protocol | 1. Let stand at room temperature to melt before use; 2. Mix gently and thoroughly, take 5μ L of this product and test sample for SDS-PAGE simultaneously. 3. The unused rainbow pre-dyeing marker shall be saved in accordance with the storage conditions. |
Operating Instructions | 1. This product can be used directly without heating. 2. Change the electrophoretic buffer and use the newly prepared gel in time to avoid affecting the electrophoretic results. 3. The product can be stably stored at 4°C for 3 months, if longer, please store at -20°C. 4. In order to avoid contamination, after opening, can be divided into small tubes according to the need, it is recommended that each tube is divided into 10μ L, -20℃ storage, take one tube at a time to use, avoid repeated freezing and thaw. |
Quality Control | Under suggested conditions, Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane. |
Overview | |
Storage Temp. | Below -15℃ for 5 years |
Properties | |
Synonym | Fetal Bovine Serum;FBS |
PH | 7.20~8.00 |
Source | High quality fetal bovine serum. |
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