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  • Fetal bovine serum (Excellent)
  • Fetal Bovine Serum;FBS
  • SKU: abs972
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    Product Details FAQ Citations(18) Video Pictures Documents
    Tips:This product is for research use only. Not for use in diagnostic prodcedures.
    Description
    Description

    This product has an excellent role in promoting cell growth and product stability, after three times 0.1 micron filtration, with sterile, no mycoplasma, no specific antibody, low endotoxin, good promoting cell growth and other characteristics.

    Appearance
    Light yellow clarification of slightly viscous liquid
    Form
    Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
    Technical Index

    Bovine immunoglobulin content: < 500mg/L;
    Protein content (g/L) : 35 ~ 45;
    Hemoglobin content (mg/L) : < 300;
    Bacterial endotoxin (Eu/ mL) : < 10;
    Osmotic pressure (mOsmol/kg) : 280 ~ 360.

    Applications
    Suitable for specific antibody requirements of higher products, more expensive cells, cell culture and closure.
    Protocol
    1. Let stand at room temperature to melt before use; 2. Mix gently and thoroughly, take 5μ L of this product and test sample for SDS-PAGE simultaneously. 3. The unused rainbow pre-dyeing marker shall be saved in accordance with the storage conditions.
    Operating Instructions
    1. This product can be used directly without heating. 2. Change the electrophoretic buffer and use the newly prepared gel in time to avoid affecting the electrophoretic results. 3. The product can be stably stored at 4°C for 3 months, if longer, please store at -20°C. 4. In order to avoid contamination, after opening, can be divided into small tubes according to the need, it is recommended that each tube is divided into 10μ L, -20℃ storage, take one tube at a time to use, avoid repeated freezing and thaw.
    Quality Control
    Under suggested conditions, Prestained Protein Ladder resolves 12 major bands in 15% SDS-PAGE (Trisglycine buffer) and after Western blotting to nitrocellulose membrane.
    Overview
    Storage Temp.
    Below -15℃ for 5 years
    Properties
    Synonym
    Fetal Bovine Serum;FBS
    PH
    7.20~8.00
    Source
    High quality fetal bovine serum.
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