Mouse CCL2/JE/MCP-1 ELISA Kit

Detection Principle:

The double antibody sandwich ELISA method was used in this experiment. Anti mouse ccl2/je/mcp-1 monoclonal antibody is coated on the microplate. Ccl2/je/mcp-1 in samples and standards will bind to the antibody fixed on the plate, and the free components will be washed away; Horseradish peroxidase labeled anti mouse ccl2/je/mcp-1 polyclonal antibody was added, and the unbound antibody was washed away; Add substrate solution (chromogenic agent), and the color of the solution is directly proportional to the bound target protein; Add termination solution; The absorbance was measured with a microplate reader.

Test Type: Double antibody sandwich method

Form: pre coated 96orifice plate

Test Sample Type: <cell supernatant, Serum, plasma

Sample loading: 100ul

Kit Components:

Precoated 96well plates, standards, anti mouse  ccl2/je/mcp-1one copy of antibody detection, dilution buffer, chromogenic solution (a, b), washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: See the instructions

Detection Range: 3.91-250 pg/ml

Recovery Range: 84-110%

Saving Method: 2-8℃

Standard Curve

Background:

CCL2/je/mcp-1 is a chemokine that binds to the receptor CCR2 and induces monocyte chemotaxis. It induces the activation of monocytes, NK cells, lymphocytes and basophils. In addition, CCL2 promotes Th2 polarization of cd4+ T cells, and CCL2 mediated recruitment of monocytes to the site of inflammation contributes to atherosclerosis, polydactylySclerosis and the severity of allergic asthma disease. Endogenous proteolytically modified CCL2, including N-terminal pyrrolidone carboxylic acid modified glutamine, downregulated activity but did not bind to the receptor.