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      abs9748

      Biotin Labeling for Avitag Protein Kit

      Avi tag protein biotin labeling kit; Biotin labeling kit, biotin label, label, Bifunctional ligase/repressor; BirA; Biotin-protein ligase
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      Tips : This product is for research use only. Not for use in diagnostic prodcedures.
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      Note: 10nmol Avi-tagged protein substrate were labeled by 2μg BirA at 37℃ for 30min. All sample lanes are loaded 4μg protein, respectively. Streptavidin has 4 biotin-binding sites, and may associate with 1 to 4 chains of the biotinylated protein. Therefore, the extent of protein biotinylation can be calculated based on the ratio of biotinylated protein to original substrate protein.

      2μg（N: non-reducing condition, R: reducing condition).

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