As we all know, fetal bovine serum is the "sweet and sweet" of the cell culture industry, and it is "favored" by the cells. In the special experimental study of some cells, dialysis fetal bovine serum can be described as exuding a "fragrance of pancakes".

 

Dialyzed fetal bovine serum (abs945)

 

The dialysis method removes small molecules with molecular weights less than 10 kDa to avoid interference with cells by exogenous small molecules. Small molecules below 10kDa include chemical small molecules, nucleotides, certain hormones, salts, low molecular weight proteins, amino acids, cytokines, glucose, residual antibiotics and other foreign molecules. It is suitable for applications such as pulsed tracing experiments, such as isotope labeling of specific small molecules and addition to culture systems to study cellular metabolism, as well as post-transfection screening applications. It is a great tool for studying proteomics, isotope labeling, cell signaling and reporter gene analysis, and reporter cell line screening.

 

1. Small molecule labeling experiments

 

The experiment is to incorporate small molecules such as nucleotides labeled with isotopes into the culture medium to label the newly synthesized DNA in the cell, generally culture for a period of time, the DNA in the cells will be isotopically labeled, and then DNA extraction, purification and other experimental analysis. In order to avoid the interference of natural nucleotides in fetal bovine serum, dialysis fetal bovine serum is required to ensure that the nucleotides incorporated into the cellular DNA are labeled nucleotides.

 

Example of a small molecule labeling experiment

 

2. Screening of positive transfected cell clones

 

Some genetically defective cell lines, such as CHO-DFGRˉ, lack dihydrofolate reductase (DHFR) and must survive in cultures supplemented with hypoxanthine, thymine, and glycine. The gene of interest and the DHFR gene we want to express can be constructed as a plasmid, then transfected into cells and cultured in medium without the above additives. Defective cells that are not transshipped into the recombinant plasmid will undergo apoptosis; Cells that are successfully transferred into the recombinant plasmid survive and form cell clones. In order to avoid the interference of hypoxanthine, thymine and glycine in fetal bovine serum, dialysis fetal bovine serum needs to be used to ensure that the complete culture medium does not contain hypoxanthine, thymine and glycine.

 

Example of screening for positive transfected cell clones

 

3. Cell platform drug screening

 

Cell-level drug screening is a drug screening model that is closer to physiological conditions, in which the model is the target cells to be designed for drug action, the cells are obtained by cell culture technology, these cells interact with candidate compounds, and the compounds are screened by measuring the ability of the compounds through detection techniques similar to biochemical level screening. In the process of drug screening, dialysis fetal bovine serum is required to avoid the interference of exogenous small molecules in the experiment.

 

Example of cell platform drug screening

 

That's all for this issue, if you have any questions about cell culture, welcome to scan the code to join our WeChat group to communicate.

 

 

This issue is recommended by Xiaoai

Catalog number	name of article	specification
abs945	Dialyzed fetal bovine serum (Premium)	500mL
abs972	Fetal bovine serum (Premium)	500mL
abs993	Exosome-free fetal bovine serum	50mL
abs943	Fetal bovine serum (activated charcoal treatment)	500mL
abs944	Fetal bovine serum (heat-inactivated)	500mL
abs936	Fetal bovine serum (for cell fusion)	500mL
abs938	Fetal bovine serum (for stem cells)	500mL
abs940	Fetal bovine serum (for 239T cells)	500mL
abs941	Fetal bovine serum (for tetracycline regulation)	500mL
abs942	Fetal bovine serum (for insect cells)	500mL

 

Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

Absin Bioscience Inc. Email: worldwide@absin.net

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