November 11,2020 Clicks:3554
|The serum is a very complex mixture of fibrin removal from plasma, the composition of which is largely known, but is still unknown, and the composition and content of the serum varies with the sex, age, physiology and nutritional conditions of the blood donors. Serum contains a variety of plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic and so on，Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding protein, provide facilitation and stretching factors to protect cells from mechanical damage, to the cultured cells in some protective role.
Fetal bovine serum should be taken from the fetus of the Caesarean section; New bovine serum is taken from newborn cows born within 24 hours; calf serum from calf born 10-30 days. Fetal bovine serum is the highest quality, because fetal cattle have not been exposed to the outside world, the serum contains antibodies, supplements and other harmful components of the cells are the least.
|Cat#||Product Name||Fill Size|
|abs972||Fetal Bovine Serum (superior grade)||125ml/ 500ml|
|abs973||Fetal Bovine Serum (superior grade)，irradiation||125ml/ 500ml|
|abs974||Fetal Bovine Serum (Standard level)||125ml/ 500ml|
|abs975||Fetal Bovine Serum (Standard level)，irradiation||125ml/ 500ml|
In addition to fetal bovine serum, Absin can also provide a variety of closed serum.
|The sample will be nonspecific and protein binding, resulting in high background, so you can use BSA or serum to close this part of the nonspecific binding, from this point of view, BSA and serum no significant difference.||②||There may be FC receptors in the sample to be tested, which can bind to the constant region of the antibody, independent of the antibody specificity, and have antibodies in the closed serum, so the serum can be used to seal off primary antibody and secondary antibody and sample FC receptors. BSA cannot close the FC receptor.|
The number of serum closed in Immunohistochemistry was more. General selection and secondary antibody the same source of closed serum(such as anti-Rabbit from the source of multiple resistance, secondary antibody for goat anti-Rabbit IgG, the closed liquid on the goat serum),The antibodies of the animals themselves in the serum can be combined with those in the tissue with a cross reaction, otherwise the background will be caused by the combination of the secondary antibody in the latter step. However, it should be noted that the closed serum can not contain the target protein, and is different from a source of resistance. In addition secondary antibody because as little as possible to choose rabbit or mouse source, because there are in the kinship and human very close, so the opportunity to produce cross reaction is relatively large, resulting in a higher background! Suitable for selection from donkey, goat, sheep, etc. secondary antibody, the background is very low.
|Cat#||Product Name||Fill Size|
|abs935||The ass/donkey serum||10ml/50ml|
|abs937||Normal mouse serum||100ml|
|abs939||Normal Monkey serum||100ml|
|◆ Fetal Bovine Serum||◆ new-born calf serum||◆ Blocking Serum|
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