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Serum

November 11,2020 Clicks:2381

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Serum

 

The serum is a very complex mixture of fibrin removal from plasma, the composition of which is largely known, but is still unknown, and the composition and content of the serum varies with the sex, age, physiology and nutritional conditions of the blood donors. Serum contains a variety of plasma proteins, peptides, fats, carbohydrates, growth factors, hormones, inorganic and so on,Its main role is to provide basic nutrients, provide hormones and various growth factors, provide binding protein, provide facilitation and stretching factors to protect cells from mechanical damage, to the cultured cells in some protective role.

Fetal bovine serum should be taken from the fetus of the Caesarean section; New bovine serum is taken from newborn cows born within 24 hours; calf serum from calf born 10-30 days. Fetal bovine serum is the highest quality, because fetal cattle have not been exposed to the outside world, the serum contains antibodies, supplements and other harmful components of the cells are the least.

 

  Products

 

    Cat#     Product Name     Fill Size
    abs972     Fetal Bovine Serum (superior grade)     125ml/ 500ml
    abs973     Fetal Bovine Serum (superior grade),irradiation     125ml/ 500ml
    abs974     Fetal Bovine Serum (Standard level)     125ml/ 500ml
    abs975     Fetal Bovine Serum (Standard level),irradiation     125ml/ 500ml

 

In addition to fetal bovine serum, Absin can also provide a variety of closed serum.
Closure is the combination of serum and nonspecific sites to eliminate non-specific staining, improve the accuracy of the target protein and reduce the background. Not all immunohistochemistry is closed by Goat Serum, Because secondary antibody is goat anti-X , so the goat serum is associated with the use of secondary antibody sources.

 

  Principle

 

①

  The sample will be nonspecific and protein binding, resulting in high background, so you can use BSA or serum to close this part of the nonspecific binding, from this point of view, BSA and serum no significant difference.   ②   There may be FC receptors in the sample to be tested, which can bind to the constant region of the antibody, independent of the antibody specificity, and have antibodies in the closed serum, so the serum can be used to seal off primary antibody and secondary antibody and sample FC receptors. BSA cannot close the FC receptor.

 

The number of serum closed in Immunohistochemistry was more. General selection and secondary antibody the same source of closed serum(such as anti-Rabbit from the source of multiple resistance, secondary antibody for goat anti-Rabbit IgG, the closed liquid on the goat serum),The antibodies of the animals themselves in the serum can be combined with those in the tissue with a cross reaction, otherwise the background will be caused by the combination of the secondary antibody in the latter step. However, it should be noted that the closed serum can not contain the target protein, and is different from a source of resistance. In addition secondary antibody because as little as possible to choose rabbit or mouse source, because there are in the kinship and human very close, so the opportunity to produce cross reaction is relatively large, resulting in a higher background! Suitable for selection from donkey, goat, sheep, etc. secondary antibody, the background is very low.

 

  Products

 

    Cat#     Product Name     Fill Size
    abs933     Goat serum     10ml/50ml
    abs935     The ass/donkey serum     10ml/50ml
    abs989     Horse serum     200ml
    abs937     Normal mouse serum     100ml
    abs939     Normal Monkey serum     100ml
    abs946     Rabbit serum     10ml/50ml
    abs947     Rat serum     100ml

 

 

 

    More Products
  ◆ Fetal Bovine Serum ◆ new-born calf serum ◆ Blocking Serum  
         

 

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