Mouse Lung Tissue Dissociation Kit: A Core Tool for Lung Cell Research

1. Tissue Pretreatment Solution: Perfuse and rinse pulmonary vessels to remove blood cells, and maintain tissue vitality after dissection.

2. Enzymatic Digestion System: The core component composed of proportionally blended enzymes:

3. Termination & Washing Buffer: Halt enzymatic reaction instantly to avoid over-digestion damage, and rinse cells for subsequent experiments.

4. Optimized Protocols: Tailored operating guidelines including optimal digestion temperature, incubation duration and tissue cutting standards.

• High Cell Viability & Yield: Maximize survival rate of functional cells.

• Complete Cell Population Coverage: Efficiently isolate hard-to-dissociate cells such as type II alveolar epithelial cells and endothelial cells.

• Excellent Reproducibility: Standardized procedures guarantee reliable and comparable experimental data.

• Time & Labor Saving: No repeated reagent formulation and condition exploration required.

1. Flow Cytometry & Cell Sorting

Prepared single-cell suspension can be directly applied to flow cytometry. Researchers can identify phenotypes, count cell populations and assess functional status including activation, proliferation and apoptosis of pulmonary immune cells like macrophages, T cells and neutrophils, as well as structural cells such as epithelial cells and fibroblasts. Fluorescence-activated cell sorting enables high-purity isolation of specific subsets for cell culture, sequencing and functional verification.

2. Single-Cell RNA Sequencing

scRNA-Seq profoundly facilitates the exploration of lung cell heterogeneity, developmental trajectory and pathological changes. Premium single-cell samples with low stress response and minimal transcriptional disturbance are ensured by the kit, supporting lung cell atlas construction, novel cell subset discovery and cell interaction mechanism study.

3. Primary Cell Culture & In Vitro Functional Assay

Isolated alveolar epithelial cells, pulmonary fibroblasts and microvascular endothelial cells can be cultured in vitro. Relevant researches cover cell proliferation, migration, fibrosis, barrier function, drug toxicity and pathogen infection models, serving as critical platforms for disease modeling and drug screening.

4. Organoid & 3D Culture

Purified lung stem cells and epithelial cells can form self-renewable lung organoids in 3D matrix gel, simulating partial physiological structure and functions of native lung tissue. It acts as powerful tool for lung development, regeneration disorder and personalized therapy research.

5. Cell Transplantation Assay

Isolated and genetically modified specific cells can be transplanted into recipient mice in stem cell and immunotherapy studies, to investigate cell homing, colonization, differentiation and therapeutic efficacy.

6. Genomics, Proteomics & Metabolomics Analysis

Uniform single-cell suspension guarantees balanced extraction of nucleic acid, protein and metabolites, reducing experimental deviation in bulk multi-omics detection.

• Match Research Objectives: Select suitable kits according to target cell types, immune cells or epithelial cells.

• Strict Protocol Execution: Thorough perfusion, precise digestion time and timely termination determine final outcomes.

• Fresh Tissue Guarantee: Use freshly harvested tissue; apply dedicated preservation solution instead of common medium for temporary storage.

• Post-dissociation Treatment: Filter cell aggregates with cell strainers and remove dead cells to improve suspension quality, especially for sensitive scRNA-Seq experiments.

• Quality Control: Assess cell viability via trypan blue staining, and verify cell composition with flow cytometry detection.

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