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March Research Renewal Season — Dual Buff Boost from Spatial Omics
May 07, 2026
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In life science research, protein localization and gene expression often exhibit temporal and spatial heterogeneity. Traditional Immunohistochemistry (IHC) and In Situ Hybridization (ISH) operate independently, making it difficult to integrate multidimensional information on a single tissue section. With the rise of Spatial Omics, researchers urgently demand technical solutions that can simultaneously profile protein phenotypes and transcript expression.
The combined solution of mIHC (Multiplex Immunohistochemistry) and GeneScope (Multicolor Single-Molecule RNA Fluorescence In Situ Hybridization) launched by Absin is tailored to meet this demand. This integrated technology enables the following analysis on the same tissue section:
- Protein Level: Multimarker colocalization (up to 7 colors)
- RNA Level: Single-molecule sensitivity detection (up to 4 targets)
- Spatial Dimension: Preserve intact tissue morphology and resolve cell-cell interaction relationships
mIHC
(Multiplex IHC)
↓
Protein Spatial Localization
Cell Type Identification
GeneScope
(RNA In Situ Hybridization)
↓
Precise Transcript Quantification
Gene Expression Validation
√ Combined Advantages
Dual Validation: Spatial Phenotype + Molecular Mechanism
Higher Reliability
1. Technical Background: Why Perform Combined Protein & RNA Detection?
In life science research, protein localization and gene expression often exhibit temporal and spatial heterogeneity. Traditional Immunohistochemistry (IHC) and In Situ Hybridization (ISH) operate independently, making it difficult to integrate multidimensional information on a single tissue section. With the rise of Spatial Omics, researchers urgently demand technical solutions that can simultaneously profile protein phenotypes and transcript expression.
The combined solution of mIHC (Multiplex Immunohistochemistry) and GeneScope (Multicolor Single-Molecule RNA Fluorescence In Situ Hybridization) launched by Absin is tailored to meet this demand. This integrated technology enables the following analysis on the same tissue section:
Protein Level: Multimarker colocalization (up to 7 colors)
RNA Level: Single-molecule sensitivity detection (up to 4 targets)
Spatial Dimension: Preserve intact tissue morphology and resolve cell-cell interaction relationships
2. Technical Principle: Synergistic Effect of 1+1>2
2.1 mIHC Technology: High-throughput Profiling of Protein Spatial Phenotypes
Absin mIHC is based on Tyramide Signal Amplification (TSA) technology. It catalyzes fluorescent dye deposition via Horseradish Peroxidase (HRP) to achieve sequential staining of multiple antibodies. Core advantages include:
| Technical Parameter | Performance Index |
|---|---|
| Detection Throughput | Simultaneous detection of 4-7 fluorescent colors |
| Sample Compatibility | Paraffin sections, frozen sections, cell slides, organoids |
| Dye Spectral Range | 480nm-770nm, full spectrum coverage |
| Signal Stability | No fading after elution, long-term storage available |
2.2 GeneScope Technology: Breakthrough in Single-molecule RNA In Situ Hybridization
Based on Rolling Circle Amplification (RCA) principle, GeneScope uses multiple pairs of Double-Linked Probes (DLPs) to specifically bind target RNA. DNA polymerase amplifies to generate hundreds of repetitive sequences for signal amplification. Compared with traditional FISH, GeneScope presents prominent advantages:
| Characteristics | GeneScope | Traditional FISH |
|---|---|---|
| Probe Design | 5-10 pairs of double-stranded probes with higher specificity | Single probe, prone to off-target |
| Signal Amplification | RCA rolling circle amplification, strong signal | No amplification, weak signal |
| Detection Sensitivity | Single-molecule level | Usually requires hundreds of copies |
| Multitarget Detection | 1-4 RNA targets per experiment | Usually 1-2 targets |
| Sample Damage | No hybridization oven required, mild conditions | High-temperature denaturation, severe tissue damage |
2.3 Technical Logic of Combined Solution
Single Tissue Section
GeneScope Staining (Round 1)
- Label chemokines (e.g. CCL22, CXCL9)
- Label cytokines (e.g. IFNG, TGFB1)
- Single-molecule sensitive quantification
- Elution and imaging
mIHC Staining (Round 2)
- Label cell type markers (e.g. CD3, CD8, CD68)
- Label functional proteins (e.g. PD-1, PD-L1, Ki67)
- Preserve morphological information
3. Application Scenarios: From Tumor Microenvironment to Neuroscience
3.1 Tumor Immune Microenvironment Analysis
In oncology research, combined protein and RNA detection reveals the dynamic relationship between immune cell infiltration and functional status:
Typical Study Design:
- mIHC Panel: CD3/CD8/CD68/PD-1/PD-L1/Ki67 (6-color panel)
- GeneScope Panel: CXCL9/CCL22/IFNG/TGFB1 (4 key chemokines/cytokines)
Scientific Value:
- Precisely locate the physical distance between PD-1+ CD8+ T cells and PD-L1+ tumor cells
- Synchronously detect IFNG mRNA expression to verify T cell functional activation
- Analyze the spatial correlation between CXCL9 expression gradient and T cell infiltration level
3.2 Neurodegenerative Disease Research
In Alzheimer’s Disease (AD) research, protein deposition and gene expression show significant temporal-spatial differences:
| Detection Dimension | Target Example | Biological Significance |
|---|---|---|
| Protein Level | Aβ42, p-Tau, Iba1, GFAP | Plaque localization, neuroinflammatory status |
| RNA Level | APP, BACE1, TREM2, IL1B | Amyloid metabolism, microglia activation |
| Combined Analysis | Protein deposition region vs gene expression region | Distinguish cause and effect |
3.3 Developmental Biology and Tissue Regeneration
In organoid research, the combined solution enables:
- Spatial Dimension: Trace the expression boundary of differentiation markers (e.g. SOX2, Nestin protein) and stemness genes (e.g. NANOG, OCT4 mRNA)
- Temporal Dimension: Construct temporal-spatial atlas of "molecular expression-morphogenesis" via serial section analysis
4. How to Order Products
mIHC Assay
Select required reagents according to standard experimental protocols for different samples, and choose dye wavelengths compatible with imaging instruments
Visit Link: https://www.absin.net/study-center/multiplex-fluorescence-staining.html
GeneScope Assay
Free design of nucleic acid fluorescence detection probes for 1-4 RNA targets plus matched kits corresponding to target quantities
Visit Link: https://www.absin.net/custom/fish.html
Contact Absin
Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.
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