A Full-Process Solution for Gastrointestinal Disease Research

In gastrointestinal disease mechanism exploration, drug screening, and scientific research, every experimental step—from animal model construction, organoid in vitro simulation, to single-cell preparation and spatial microenvironment analysis—requires stable, efficient, top-journal-validated reagents and comprehensive protocol support. Absin, as the core brand under UniVenture deeply engaged in the life sciences, addresses the core needs of gastrointestinal disease research by developing an integrated full-process solution covering "Colitis Model Construction—Intestinal Organoid Culture—Intestinal Tissue Dissociation—mIHC Multiplex Immunofluorescence Histochemistry," accompanied by top-journal equivalent reagents that align with experimental scenarios throughout the process. This empowers researchers to efficiently advance their projects, rapidly publish high-impact articles, and comprehensively covers core research directions including Ulcerative Colitis and Colorectal Cancer.

I. Colitis Model Construction and Mechanism Research

Ulcerative Colitis (UC) represents a central focus in gastrointestinal inflammation research. The Dextran Sulfate Sodium (DSS)-induced UC mouse model serves as a classic experimental paradigm in this field, effectively mimicking the intestinal inflammatory characteristics and intestinal barrier damage observed in human ulcerative colitis. Absin provides mature model construction reagents and complete experimental protocols, enabling full-process coverage from model induction to mechanism validation, including dynamic body weight monitoring in mice, colon length measurement, intestinal pathological section H&E staining scoring, as well as molecular and functional validation such as inflammatory cytokine expression and intestinal barrier permeability assessment—perfectly suited for inflammation mechanism exploration, drug screening, and therapeutic efficacy evaluation research scenarios.

DSS+IGF2 Model: Barrier Disruption · Inflammation Induction · Protection Validation — Ulcerative Colitis Mechanism and Drug Screening<sup>[1]


Gut-Brain Interaction Model    Intestinal Pathology · Behavioral Analysis · Molecular Mechanisms — UC Complications and Therapeutic Targets[2]</sup>

Recommended Products

Literature Support

【1】Eur J Pharmacol. 2023 Dec 5:960:176122. doi: 10.1016/j.ejphar.2023.176122. IF:5

【2】Redox Biol. 2024 Nov:77:103370. doi: 10.1016/j.redox.2024.103370. IF:5.7

II. Intestinal Organoid Culture Protocols

Organoid technology represents a revolutionary tool for gastrointestinal disease research, capable of highly simulating the physiological structure, cellular composition, and pathological characteristics of the intestine in vitro. It is particularly applicable to heterogeneity studies in colorectal cancer, drug sensitivity testing, and intestinal physiological development research. Absin provides a comprehensive series of intestinal organoid culture products covering human, mouse, and bovine sources to meet diverse species and research requirements, including colorectal cancer organoid culture kits and normal intestinal organoid culture media. With optimized formulations and convenient operation, these products enable efficient cultivation of high-activity, morphologically stable organoids, perfectly adapted to multi-scenario gastrointestinal disease research.

                                     
                                                                               Colorectal Cancer Organoids Cultured Using Human Colorectal Cancer Organoid Culture Kit (abs9445)

Recommended Products

Literature Support

【1】Cancer Res. 2026 Feb 9. doi: 10.1158/0008-5472.CAN-25-2586. IF:16.6

【2】Gut Microbes. 2026 Dec 31;18(1):2639216. doi: 10.1080/19490976.2026.2639216. IF:11

【3】Acta Pharm Sin B. 2024 Jul;14(7):2959-2976. doi: 10.1016/j.apsb.2024.04.024. IF:14.6

III. Intestinal Tissue Dissociation and Single-Cell Preparation

Intestinal tissue possesses a complex structure containing abundant matrix components and diverse cell types. Efficient and gentle tissue dissociation is critical for the success of downstream experiments including flow cytometry, single-cell sequencing, and cell sorting. Absin provides a one-stop solution from sample preservation, tissue dissociation to cell filtration and purification. With gentle reagent formulations, intestinal tissue can be rapidly dissociated into high-viability single-cell suspensions, maximally preserving cellular activity and phenotype while effectively preventing cell damage—perfectly adapted for isolation and analysis of intestinal immune cells and tumor cells, providing reliable assurance for single-cell level research in gastrointestinal diseases.

Flow Cytometry Cell Viability Staining Results of Single-Cell Suspension from Mouse Intestinal Tissue Processing

Recommended Products

Literature Support

【1】Adv Sci (Weinh). 2024 Dec 24;12(7):2409537. doi: 10.1002/advs.202409537. IF:17.5

【2】J Exp Clin Cancer Res. 2023 Dec 15;42:339. doi: 10.1186/s13046-023-02890-z. IF:11.3

【3】Int J Biol Sci. 2025 May 27;21(8):3555-3572. doi: 10.7150/ijbs.107837. IF:10

IV. mIHC Multiplex Immunofluorescence Histochemistry Solution

The intestine represents the body's largest immune organ. Spatial distribution of cells, multi-target co-localization, and cell-cell interactions constitute the core of spatial mechanism research in gastrointestinal diseases (particularly colorectal cancer and inflammatory bowel disease). Absin provides four-color to seven-color Multiplex Immunohistochemistry (mIHC) kits with universal secondary antibodies for mouse and rabbit, compatible with human, mouse, and other species' intestinal tissue samples. These enable simultaneous multi-target staining without dewaxing or counterstaining, offering convenient operation and stable signals for clear visualization of intestinal tissue spatial structure, immune cell distribution, and tumor-immune interactions—providing powerful tools for spatial omics research in gastrointestinal diseases.

Human Intestinal Tissue Tertiary Lymphoid Structure (TLS) mIHC Results
CD20⁺ B cells, CD21⁺ follicular dendritic cells, CD4⁺ T cells exhibit organized spatial distribution resembling secondary lymphoid organs, indicating functional potential for B cell activation, antibody production, and T-B cell collaborative immune responses

Mouse Colorectal Cancer 7-Color mIHC Results
Tumor epithelium, immune cells, and stromal cells exhibit organized spatial arrangement within the tumor microenvironment, indicating functional potential for strong immunosuppression, pro-angiogenesis, stromal remodeling, and nerve-tumor interactions that promote tumor progression.

Recommended Products

Literature Support

【1】Signal Transduct Target Ther. 2025 Oct 7;10:331. doi: 10.1038/s41392-025-02426-1. IF:52.7

【2】Cancer Cell. 2025 Jan 13;43(1):122-143.e8. doi: 10.1016/j.ccell.2024.12.006. IF:44.5

【3】Cell. 2025 Mar 3. doi: 10.1016/j.cell.2025.02.002. IF:42.5

Brand Strength and Partnership Assurance

Absin has consistently adhered to the core philosophy of "Quality Research Reagents, Choose Absin," deeply engaged in the life sciences for many years. All products undergo rigorous quality testing and experimental validation, with cumulative product literature publications exceeding 18,000, covering the full process of gastrointestinal disease research. Leveraging UniVenture's powerful supply chain and technical support system, Absin provides researchers with comprehensive experimental solutions—from reagent selection, experimental operation guidance to technical problem resolution—ensuring full support throughout the journey to help researchers efficiently advance their projects and rapidly publish high-impact articles.

Quality Research Reagents, Choose Absin! Premium Reagents for Daily Research Success!