Panoramic solution for cell function detection

Covering the four dimensions of vitality and proliferation/apoptosis/metabolism/stress · Enabling drug development and mechanism research.

 

High sensitivity detection · High throughput compatibility · Full process verification · 15,000 literature citations

 

1. Cell viability and proliferation detection

 

Cell viability and proliferation are the basic indexes to evaluate the physiological state of cells and the effects of drugs. Aibixin provides a variety of detection kits, covering different detection methods such as bioluminescence, colorimetry and imaging, which can meet different experimental scales and sensitivity needs, and are widely used in drug screening, cytotoxicity assessment and other scenarios.

 

Item number	Product name	Specifications	peculiarity
abs50065	CTG-L ™ 2D cell viability test kit	10-1000mL	Bioluminescence, one-step method, high sensitivity, wide linear range, 2h half-life, suitable for high-throughput detection, comparable to imported P * * brands
abs50003	CCK-8 kit	500T/5000T	Cell viability/proliferation, one-step method, absorbance detection, supports high throughput, 300 + citations
abs50050	EdU-488 Cell Proliferation Detection Kit (Imaging)	200T	Clicreaction, more sensitive than BrdU, in vitro + in vivo full support
abs50010	MTT cell proliferation detection kit	500T	Two-step method, the absorbance is measured after the product is dissolved in solvent, and the cell viability/proliferation is detected, classical method
abs50011	MTS cell proliferation detection kit	500T/2500T	MTT upgrade method, no solvent required, one-step detection of cell viability/proliferation, classical method

 

 

abs50003 application case:

Cell viability of hGFs cells co-cultured with PolyLA-Na/PolyLA patch for 1 and 3 days (n = 4);

Cui C, Mei L, Wang D, Jia P, Zhou Q, Liu W. Nat Commun. 2023 Nov 24; 14 (1): 7707. doi: 10.1038/s41467-023-43571-x.

 

 

abs50065 application case:

HepG2 cell line, after treatment with different concentrations of STSP, compare abs50065 and imported P * * company products respectively. EC50, SD and CV were calculated on 10/20/30/40/50/60/90/120/150/180 minutes after adding the detection reagent. Data, the performance of Aibixin products is comparable to that of imported P * * products.

 

2. Apoptosis detection

 

Apoptosis is a process of programmed cell death, which is closely related to the occurrence and development of diseases. Aibxin Apoptosis Detection Kit can detect apoptosis status from different levels, including early mitochondrial membrane potential changes, apoptosis-related key protein (Caspase enzyme) activity and cell staining characteristics, etc., providing a powerful tool for apoptosis mechanism research and drug development.

 

Item number	Product name	Specifications	peculiarity
abs50001	Annexin V-FITC/PI Apoptosis Detection Kit	25-100T	Classical double staining, which can distinguish early/late apoptotic cells, fully supported by imaging + flow cytometry detection, 300 + citations
abs50198	CAS-GT ™ Caspase3/7 Apoptosis Detection Kit	2.5-100mL	Bioluminescence, one-step method, high sensitivity, wide linear range, 2h half-life, suitable for high-throughput detection, comparable to imported P * * brands
abs50016	Mitochondrial membrane potential detection kit (JC-1)	100T	Early indicator of apoptosis, high sensitivity detection, 30 + citations

 

 

abs50001 application case:

Flow cytometry analysis showed increased apoptosis in Cancer-associated fibroblasts (CAFs) after S100A13 knockdown.

Xia L, Guo X, Lu D, Jiang Y, Liang X, Shen Y, Lin J, Zhang L, Chen H, Jin J, Luan X, Zhang W. Cell Commun Signal. 2025 Jan 28; 23 (1): 51. doi: 10.1186/s12964-025-02049-7.

 

 

abs50198 application case:

4 × 10^5/mL of HeLa cells were seeded on 96-well white plate clear bottom plates, 100 µ l/well.

After 24 hours, 3-fold serial dilution of STSP was added, and after incubation at 37 °C with 5% CO2 for 6 hours, Caspases 3/7 activity was detected according to the instructions of abs50198 cell apoptosis detection reagent.

The figure above shows the dose curve of reading the fluorescence value 30 min-130 min after adding the detection reagent, and the table shows the calculated EC50 value.

 

3. Cell metabolism-related detection

 

Cellular metabolism is a core process that maintains cellular function, and its abnormalities are related to a variety of diseases. Albixin metabolism-related kit focuses on key metabolic indicators such as glucose uptake, lactate production, NAD +/NADH balance and ATP level, which can quantitatively analyze the state of cellular energy metabolism and assist metabolic mechanism research and drug development for metabolism-related diseases.

 

Item number	Product name	Specifications	peculiarity
abs580267	Glucose Uptake Fluorescence Kit	96 T	Fluorescence detection, high sensitivity, wide linear range, quantitative detection of cellular exogenous glucose uptake
abs580160	Lactic acid (LA) assay kit	96 T	Colorimetric method, high cost performance, quantitative detection of lactic acid content changes in samples
abs90116	NAD +/NADH detection kit (WST-8 method)	96 T	Colorimetric method, two-step determination of NADH and NAD + content, high sensitivity, needs to be used together with quantitative detection of total protein in the sample (abs9232)
abs580117	ATP Assay Kit	96 T	Bioluminescence, high sensitivity, wide linear range, accurate quantification of intracellular ATP expression level, assessment of cellular energy metabolism

 

4. Cytotoxicity and stress detection

 

When cells are stimulated by the outside world, they will produce toxic reactions and stress responses, which are an important basis for evaluating drug safety and cellular adaptability. The following kits can detect indicators such as reactive oxygen species, oxidized glutathione, lactate dehydrogenase and kinase-related toxicity, and comprehensively reflect the stress state and damage degree of cells.

 

Item number	Product name	Specifications	peculiarity
abs580232	Reactive oxygen species ROS detection kit (green fluorescence)	96 T	Fluorescent probe detection, high sensitivity, high throughput, imaging + flow detection full support
abs580264	Oxidized glutathione (GSSG) content kit (microplate method)	96 T	Colorimetric method, cost-effective, two-step accurate measurement of GSSG content in samples and evaluation of oxidative stress levels
abs580274	Lactate dehydrogenase (LDH) cytotoxicity test kit	480T	Colorimetric method, cost-effective, supporting high throughput, classical principle to assess cytotoxicity by LDH activity
abs50197	ADP-KTM Kinase ADP Detection Kit	400T/1000T	Bioluminescence method, high sensitivity, wide linear range, direct evaluation of kinase inhibitor drug toxicity, supporting mM ATP level reaction system

 

Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

Absin Bioscience Inc. Email: worldwide@absin.cn

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