EAE model antigen selection strategy

experimental autoimmune encephalomyelitis (EAE) is the most common animal model of multiple sclerosis (MS), with many clinical and pathophysiological features. EAE models are diverse and reflect diverse clinical, immunological, and histological features of human MS. The mouse model of EAE by active induction is the most easily inducible model with robust, replicable results.

 

The induction modes of EAE include: active induction [1] and passive induction [2]. Active induction refers to the emulsification of myelin antigen mixed with complete Freund's adjuvant (CFA), the immunization of susceptible animals with the emulsifier, and the combination of auxiliary components such as pertussis toxin (PTX) to enhance the immune response, thereby inducing EAE model. Myelin antigens mainly include Myelin Oligodendrocyte Glycoprotein (MOG), Myelin Proteolipid Protein (PLP) and Myelin Basic Protein (MBP).

 

 

1. MOG (35-55) (abs815889)

 

Species: C57BL/6 mouse

 

Advantages:

① It is very suitable for studying the occurrence and development of EAE and testing potential treatment methods.

② The group size can be smaller (10-12 mice/group), because EAE develops in more than 90% of mice.

③ The first wave of EAE symptoms usually lasts for 7 days, then partial recovery, followed by chronic paralysis, and it takes longer to observe the differences between experimental groups.

 

Disadvantages:

① It is not suitable for studying EAE recurrence and targeted B cell therapy. The antigen does not always induce the production of antibodies.

② Most of the C57BL/6 mice developed chronic EAE.

③ It has been reported that EAE induced by MOG (35-55) or MBP (1-11) (abs45131577) peptides is not related to the presence of B cells [3, 4]. Therefore, peptide-induced EAE models may not be suitable for testing therapeutic drugs against B-cell depletion or impaired B-cell function.

 

2. MOG (1-125) (abs05465)

 

Species: C57BL/6 mouse

 

Advantages:

① Recommended for testing therapies aimed at impairing B cell function. Consistent anti-MOG (1-125) antibody production was induced.

② It has been reported that MOG (1-125)-induced EAE is likely to be a good model for testing therapeutic drugs targeting B cells.

 

Disadvantages:

① Antigens are expensive.

② Spontaneous recovery was greater than when MOG (35-55) was used as antigen. Therefore, the treatment window at the end of the study was smaller compared to the use of MOG (35-55).

 

3. [Ser140]-PLP (139-151) (abs45152261)

 

Species: SJL mouse

 

Advantages:

① It is very suitable for studying EAE recurrence. Most mice will recover from the first wave of EAE, 50% to 80% of mice will recover.

② The incidence of EAE was 90% to 100%, and the onset of EAE was synchronous.

③ EAE can be induced with or without pertussis toxin (abs42024900).

 

Disadvantages:

① The symptoms of the first wave of EAE were short (2-5 days in most mice), and then recovered spontaneously, resulting in a small treatment window at the end of the first wave of EAE.

② It is recommended that the scale of 15-20 mice be used for recurrence studies, because the recurrence rate is 50%-80%.

③ If the first wave (infection) and recurrence are tracked at the same time, the validity period of the model is 40 days or longer.

④ If pertussis toxin (abs42024900) is administered, the recurrence rate and severity will be reduced.

 

4. PLP (139-151) (abs45153151)

 

Species: SJL mouse

 

Advantages:

Compared with [Ser140]-PLP139-151, it induced more severe EAE and did not reduce the recurrence rate. Pertussis toxin (abs42024900), if administered to enhance the severity of EAE, tends to reduce the recurrence rate.

 

Disadvantages:

Same as [Ser140]-PLP139-151. Furthermore, the EAE can be too severe.

 

5. MBP (69-88) (abs45152786)

 

Species: Lewis rat

 

Advantages:

Disease onset and severity were consistent. The study duration was short (usually 18 days).

 

Disadvantages:

① Rats are larger than mice and require more compounds.

② Because rats will recover spontaneously, the treatment window is narrow at the end of the first wave of EAE.

③ Lewis rats do not have demyelination phenomenon and do not experience the chronic phase of EAE.

 

In addition, we provide high-purity (HPLC ≥ 98%), high-specificity myelin protein peptide customization services, covering a full range of EAE/MS research antigens to meet the individual needs of your disease research. Peptide customization, peptide synthesis, synthetic peptides.

 

References:

[1] Stromnes IM, Goverman JM. Active induction of experimental allergic encephalomyelitis. Nat Protoc. 2006;1(4):1810-1819.

[2] Stromnes IM, Goverman JM. Passive induction of experimental allergic encephalomyelitis. Nat Protoc. 2006;1(4):1952-1960.

[3] Wolf SD, Dittel BN, Hardardottir F, Janeway CA Jr. Experimental autoimmune encephalomyelitis induction in genetically B cell-deficient mice. J Exp Med. 1996;184(6):2271-2278.

[4] Lyons JA, San M, Happ MP, Cross AH. B cells are critical to induction of experimental allergic encephalomyelitis by protein but not by a short encephalitogenic peptide. Eur J Immunol. 1999;29(11):3432-3439.

 

EAE Model Product Recommendation

Item number	Product name	Specifications
abs42024900	Whooping cough toxin	50μg
abs815889	MOG(35-55)	5mg
abs05465	MOG(1-125)	500μg
abs45152261	[Ser140]-PLP (139-151)	5mg
abs45153151	PLP (139-151)	1mg
abs45152963	PLP (178-191)	5mg
abs45152786	MBP (69–88)	1mg
abs45131577	MBP(1-11)	5mg
abs9270	Freund's complete adjuvant	10mL

 

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