Proteomics Sample Preparation Solutions-From Protein to Peptide

 

Proteomics sample preparation can provide methodological support for studying protein structure, interaction mapping, studying epigenetics, finding disease markers and expanding tumor screening at the protein level; Before using the mass spectrometer, a set of mature and stable sample preparation methods can be explored to ensure the quality and consistency of the identification results.

 

Sample Type

 

Cell samples: including suspended cells and adherent cells, etc., appropriate lysis methods and extraction methods should be selected according to cell types.

 

Tissue samples: cover animal tissues (such as heart, liver, spleen, kidney, lung, etc.), plant tissues (such as leaves, flowers, fruits, seeds, roots, bark, etc.) and mollusks (such as schistosomiasis, trichinella spiralis, etc.), which need to be properly treated, such as cutting, washing, quick freezing, etc., to ensure the integrity of samples and the efficiency of protein extraction.

 

Liquid samples: There are plasma or serum (low-abundance protein needs to be enriched first), saliva, tears, urine, milk, cerebrospinal fluid, ascites, etc. The collection, processing and preservation methods of different liquid samples have their own characteristics.

 

 

Plasma Low Abundance Protein Enrichment Magnetic Bead Kit

 

Based on the method of immunomagnetic bead capture, the circulating proteins in plasma samples are enriched, which can reduce the effect of high-abundance proteins on the signal coverage of low-abundance proteins in downstream proteomics detection, thereby improving the signal strength of low-abundance proteins, and improving the repeatability of protein identification quantity and quantification.

 

 

Proteomics sample preprocessing kit

 

For the treatment of trace amounts of protein (5-100μg), preprocessed samples can be quickly prepared and can be used for subsequent mass spectrometry analysis.

 

Taking 50μg 293T cell protein as an example, the identification amounts of protein and peptide segments are shown in the figure

 
Phosphopeptide protein (peptide) extraction kit

 

Polymer-based affinity capture of immobilized metal ions with superior phosphorylated proteome coverage and recovery.

 

Take 100μg 293T peptide as an example, the protein, phosphorylated peptide and phosphorylation site identification amounts are shown in the figure

 

Proteomics Sample Preparation

 

Applications	Item number	Name
Proteolysis + alkylation	abs90136	Protein lysate
Enzymatic hydrolysis	abs9916	Recombinant trypsin (mass spectrometry grade)
	abs90145	Recombinant lysyl endonuclease (mass spectrometry grade)
Desalination	abs90144	Polypeptide desalting cartridge
Trace protein (5-100 μg) pretreatment	abs50141	Proteomics sample preprocessing kit
Protein phosphorylation enrichment	abs9917	Phosphopeptide protein (peptide) extraction kit
Low abundance enrichment of plasma samples	abs9918	Plasma Low Abundance Protein Enrichment Magnetic Bead Kit

 

 

Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

Absin Bioscience Inc. Email: worldwide@absin.cn

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