Time flies, and the 14th organoid training course from September 26, 2024 to September 27, 2024 has been successfully concluded. The training adopted small class teaching (6 people) to better guide each trainee's practical operation, and demonstrated the entire operation process of organoid technology from primary culture, subculture, cryopreservation, and recovery, and spent a pleasant learning time together.

Next, let's take a look back at the highlights.

 

Explanation of the application of organoid technology

Organoid construction process

The course is mainly in the form of a combination of theoretical classroom and on-site teaching, which closely combines theory and practice. First of all, Dr. Li, our head of organoids, will give a lecture on the application of organoid technology, share some of the achievements of our Abison, and discuss the development and cutting-edge information of organoids in recent years. Then, Fang Bo mainly shared the knowledge related to the practical operation of organoids, from the whole process and precautions of surgical material collection, primary culture, subculture, cryopreservation and resuscitation experiment, and explained the identification methods of organoids, which can be judged from the tissue level using histochemical identification methods and molecular biology sequencing technology.

 

Practical explanation of organoids

 

Before the experiment began, the students were allowed to observe the brightfield diagrams of different types of samples, so that they could better judge the cultivation of their own samples during subsequent training. Because the selection and digestion of each sample are different, we specially displayed a variety of samples such as mouse small intestine, colon, lung, liver, and stomach. Fang Bo demonstrated the entire operation process and precautions of primary culture, subculture, and cryopreservation of organoids.

Next, let's enjoy this issue of organoid videos and beautiful photos!

Human liver cancer organoids

Human lung cancer organoids

Human intestinal cancer organoids

Mouse small intestinal organoid P0D1

 

Mouse lung organoid P0D1

The above is the beautiful photos of organoids in this issue, and we took a group photo at the end!

 

Group photo

This is the end of this issue of the Abisure Practical Camp, and each of our students has mastered the key points of organoid culture. The performance of each student is very good, actively participate in the process and discuss the problems encountered, and the partners guide each other, help each other and make progress together. Finally, I would like to thank every student who has come from thousands of miles away, organoid technology allows us to gather here, learn from each other, and make progress and development together!

National Day Product Activities:

 

Organoid culture product recommendation

Catalog number	name of article	specification
abs9495	Matrigel (low factor, no phenol red)	1.5mL*4/1.5mL*8
abs9523	Human bowel cancer organoid medium	500mL
abs9524	Human gastric cancer organoid medium	500mL
abs9525	Human lung cancer organoid medium	500mL
abs9788	Human hepatocholangiocarcinoma organoid medium	500mL
abs9780	Human bladder cancer organoid medium	500mL
abs9782	Human esophageal cancer organoid medium	500mL
abs9639	Human cervical cancer organoid medium	500mL

 

 

Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

Absin Bioscience Inc. Email: worldwide@absin.net

Follow us on Facebook: Absin Bio