Want to get a high-quality single-cell suspension quickly? Try this Absin automatic single-cell suspension preparation instrument that is popular in the scientific research circle, and teach you from sample to data step by step!
Why choose a fully automated preparer?
1) Time-saving and labor-saving: the traditional manual operation takes 2h, but now it is automatically completed in 30min;
2) Reliable results: 85%+ cell activity, 90%+ yield, farewell to cell debris interference;
3) One machine for multiple purposes: tumor tissue, lymphoid tissue, and peripheral blood samples can be processed;
4) Novice-friendly: 6 sets of preset programs, as simple as selecting the photo mode on the phone;
5) International standard: DIN method design, low noise, constant temperature control, stable performance.
The experimental process is a five-step process
Step 1: Sample preparation
1) Cut the fresh/frozen tissue sample into 5-10mg pieces (about the size of sesame seeds);
2) Add digestive enzymes and zirconia grinding beads;
3) Sample size support: 5mg to 1g (tissues as small as rice grains can also be processed).
Step 2: Parameter settings
1) Intelligent mixing: the instrument automatically mixes the sample with high-density zirconia magnetic beads and collagenase/hyaluronidase;
2) Select a preset program (customizable):
a. Tumor mode: strong shear at constant temperature 37 °C;
b. Nerve cell mode: gentle shaking at 28 °C;
c. Plant mode: 42°C for wall breaking.
3) Advanced Settings (Optional):
a. Precise temperature control (22-56°C);
b. Oscillation frequency adjustment (500-4000 rpm)
Step 3: One-click start
1) The metal bath temperature control cabin began to work (8 tubes were processed at the same time);
2) Countdown 30min:
a. First 10min: Isothermal enzymatic hydrolysis to soften tissue;
b. Medium 15min: high-frequency shear of zirconia beads;
c. After 5 min: Digestion is automatically terminated and filtered.
Step 4: Harvest the suspension
1) Cell yield starts at 90%: It is easy to obtain live cells from tens of millions to millions
2) Complete three key steps:
a. Lysis of red blood cells (for blood samples);
b.70μm filter filter (automatically completed by integrated pipeline);
c. Activity detection (APP displays concentration and viability synchronously).
Laboratory Cases
Product technical advantages
1) Metal bath + shearing double engine: constant temperature digestion and physical shearing are carried out simultaneously, and the efficiency is doubled;
2) Fool-style operation: the whole process from tissue block to suspension is automated, and novices can also publish top journals;
3) Supporting artifact: Zirconia grinding beads are specially designed for fragile cells to avoid metal contamination.
Frequently Asked Questions
Q: Do I have to use matching grinding beads?
A: It is recommended to use ABS7380 zirconia beads (1 bottle will be given when you place an order), other brands may damage the cells.
Q: Can I treat tissues with severe fibrosis?
A: Select "Tumor Strength Mode" + matching type IV collagenase, and liver cirrhosis samples can be easily dissociated.
Q: Is the instrument noisy?
A: The measured < in the laboratory is 55 decibels (equivalent to the sound of an office printer).
Related reagents
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Catalog number
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Name
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Applicable Organizations
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abs7380
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4mm grinding beads (zirconia)
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Zirconia material, used with grinding instrument and single cell suspension preparation instrument
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abs7381
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7mm Grinding Beads (Zirconia)
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Zirconia material, used with grinding instrument and single cell suspension preparation instrument
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abs50090
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Mouse tumor tissue dissociation kit
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Specially formulated for tissue-specific isolation
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abs50091
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Mouse liver tissue dissociation kit
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abs50092
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Mouse lung tissue dissociation kit
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abs50093
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Mouse intestinal tissue dissociation kit
|
|
abs50094
|
Mouse heart tissue dissociation kit
|
|
abs50095
|
Mouse kidney tissue dissociation kit
|
|
abs50096
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Mouse pancreatic tissue dissociation kit
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|
abs50097
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Mouse spleen tissue dissociation kit
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|
abs50098
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Mouse brain tissue dissociation kit
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abs50099
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Human Tumor Tissue Dissociation Kit
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abs9482
|
Tissue dissociation solution
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It is used for the separation of brain tissue, heart, spleen, liver, kidney, pancreas, intestine, gastric mucosal tissue, gallbladder, tumor tissue and other tissue cells
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abs47048000
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Collagenase type I
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Isolation of breast tissue cells
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abs47048001
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Collagenase type II
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It is used for the isolation of pancreatic tissue cells
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abs47048002
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Collagenase type III
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|
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abs47048003
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Collagenase type IV
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|
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abs47048004
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Collagenase type V
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|
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abs47048005
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Collagenase NB4 standard level
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|
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abs50055
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Tissue digestion kit
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It is used for the isolation of liver cancer tissue, lung cancer tissue, breast cancer tissue, ovarian cancer tissue and glioma tissue
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abs9868
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ACF mesenchymal stem cell isolate
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It is used to isolate mesenchymal stem cells derived from different tissues (umbilical cord, adipose, placenta).
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abs9870
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Human adipose stem cell isolate
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Dedicated to the isolation of adipose-derived mesenchymal stem cells
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Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.
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Absin Bioscience Inc.
Email: worldwide@absin.net
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