Transfection is a technique for introducing exogenous genes (DNA or RNA) into cells. With the development of gene and protein functional research, transfection has become a fundamental method frequently used in laboratories.

Based on the duration of the introduced nucleic acid within the host cells, it can be divided into transient transfection and stable transfection.

Transient Transfection: Refers to the process of introducing a constructed plasmid into mammalian cells through certain methods, where the exogenous gene on the plasmid is not integrated into the cell's own genome. The expression of exogenous genes in cells transiently transfected is limited in time, usually lasting only a few days until the exogenous gene is lost due to various factors during cell division. It is often used for the analysis of promoters and other regulatory elements.

Stable Transfection: The exogenous DNA is integrated into the cell genome or retained in the cell as an episomal plasmid. Unlike transient transfection, stable transfection can integrate into the host genome, so the transfected DNA will be retained in the genomes of the transfected cells and their progeny.

Today, we will introduce the transfection reagents from domestic company absin.

一、Plasmid DNA Transfection

Lipofect5000 Plasmid Transfection Reagent. It is a high-performance universal nucleic acid transfection reagent developed based on the performance of similar foreign products, suitable for transfecting plasmid DNA into mammalian cells.

二、RNA Transfection

1、Cells 
abs60621 easyLNP™ Plus Universal RNA Cell Transfection Kit

Features:
1.Unique transfection mechanism: The lipid nanoparticle mixture with RNA achieves transient encapsulation, utilizing the unique transmembrane transport and endosomal escape mechanism of LNP to efficiently deliver RNA.
2.Convenient operation: After forming a complex with RNA, this product does not require standing incubation and can be immediately transfected, shortening the experimental time. No medium change is needed after transfection, making the operation simple.
3.Safety: This product is filtered through a 0.2μm micropore filter, has stable quality, uses new biodegradable lipid materials, has very low cytotoxicity, and does not contain ethanol to avoid cell inactivation caused by ethanol.

Applications:

Suitable for common cell mRNA and short-chain RNA transfection.

Using a 96-well plate, transfect 100ng of eGFP circRNA per well into cells, and take fluorescence photographs after 24 hours.

abs60623 easyLNP™ mRNA In Vivo Transfection Kit (Local Action)

Features：

Applications：

Suitable for local mRNA transfection in animals.

Intratumoral Injection: Achieving Efficient Expression of RNA in Tumor Tissue