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      HomeProduct ApplicationAbsin Bioluminescence Detection Solution — Comprehensive Product Portfolio, Benchmarked Against Imported Gold Standards

      Absin Bioluminescence Detection Solution — Comprehensive Product Portfolio, Benchmarked Against Imported Gold Standards

      Bioluminescence is a chemical reaction within living organisms. Its principle involves specific enzymes catalyzing the oxidation of substrates to produce excited-state intermediates. When these intermediates return to their ground state, they release photons.
      Based on this principle, a series of bioluminescence-based detection reagents have been developed and are widely used in the field of life sciences. These reagents generally have the following advantages: simple operation, high sensitivity, wide dynamic range, low background interference, and suitability for high-throughput and automated scenarios.
      Abcam has developed a series of high-performance and cost-effective detection products based on bioluminescence methods to assist a wide range of scientific research clients.

       

      1. Cell Viability & Apoptosis Detection Kits

      Abcam's CTG-LTM and CTF-LTM series products are based on the bioluminescence detection principle of luciferase and the detection principle of live-cell protease activity to measure cell viability. The CAS-GTM product utilizes the activity of Caspase 3/7 enzymes to design corresponding bioluminescent substrates for more sensitive detection of early cell apoptosis. The entire product line is fully comparable to the industry gold standard P* brand, with advantages such as simple operation, wide linear range, high sensitivity, stable signal, and suitability for high-throughput automated scenarios.



      Fig. 1. Schematic diagram of the principle of cell viability detection using the luciferase method in the CTG-LTM series


      Fig. 2. Schematic diagram of the principle of live-cell protease activity detection in the CTF-LTM series


      Fig. 3. Schematic diagram of the principle of caspase 3/7 enzyme activity detection in the CAS-GTM series

      Product Number

      Product Name

      Specification

      Features

      abs50065

      CTG-L™ 2D Cell Viability Detection Kit

      10 & 100 & 1000 ml

      Upgraded, stronger signal, supports large packaging

      abs50059

      2D/3D/Organoid ATP Viability Assay Kit

      10 & 100 ml

      Optimized for organoid

      abs50190

      CTG-L™ 3D Cell Viability Detection Kit

      10 & 100 ml

      Optimized for organoid, better signal

      abs50189

      CTG-L™ 2D Cell Viability Detection Kit 2.0

      100 & 500 ml

      Improved stability

      abs50193

      CTF-L™ 2D Fluorescent Cell Viability Detection Kit

      10 & 50×2 ml

      Ex380/Em510, luminescence method, non-destructive

      abs50198

      CAS-G™ Caspase3/7 Cell Apoptosis Detection Kit

      2.5 & 10 & 100 ml

      Luminescence method for detecting Caspase enzyme activity

       

      2. Luciferase Reporter Gene Assay

      The luciferase reporter gene assay system has become an indispensable tool in life sciences research due to its high sensitivity, specificity, and ease of operation. It is widely used in various fields such as gene expression, signal transduction, protein-protein interactions, cell biology, oncology, microbiology, drug development, gene therapy, biosensor development, and in vivo imaging.
      Abcam's luciferase reporter gene assay kits are of excellent performance and comprehensive coverage. They include commonly used firefly and Renilla luciferase detection systems and their combinations into dual-luciferase detection kits. There are also smaller molecule Nano luciferase detection systems with less interference. For the most widely used firefly luciferase detection reagent, we have developed several different reagents with longer signal half-life, stronger signal, and better storage stability to meet the needs of different customers.


      Fig. 4. Schematic diagram of the detection results for the DUG-LTM Dual-Luciferase Reporter Assay Kit

      Plant HEK293 cells at a confluence of 60% into a white 96-well plate with a clear bottom at a volume of 100 µl per well. On the second day, transfect the cells with 0.2 µg of DNA of two plasmids, pCMV-Fluc and pCMV-Rluc, per well. After 48 hours, measure the activity of the dual luciferases according to the product manual. The experimental data represent the average of four wells. Group A: The relative light units (RLU) after adding reagent component R1; Group B: The RLU after adding R2 buffer; Group C: The RLU after adding R2 substrate.

      Product Number Product Name Specification Features

      abs60672

      STG-L™ Stable Luciferase Reporter Gene Detection Kit

      100 & 1000 ml

      Stable type, half-life exceeds 4 hours

      abs60673

      BRG-L™ High Luminosity Luciferase Reporter Gene Detection Kit

      100 & 1000 ml

      High brightness, stronger signal

      abs60674

      ONG-L™ One-luc Luciferase Reporter Gene Detection Kit

      100 & 1000 ml

      Good chemical stability, no decay for 4°C 5 days

      abs60675

      REG-L™ Renilla Luciferase Reporter Gene Detection Kit

      10 & 100 ml

      Detection reagent for Renilla luciferase system

      abs60676

      DUG-L™ Dual Luciferase Reporter Gene Detection Kit

      10 & 100 ml

      Detection of firefly & Renilla dual luciferase reporter genes

      abs60677

      NAG-L™ NLuc Luciferase Reporter Gene Detection Kit

      10 & 100 ml

      Detection reagent for Nano luciferase system

      abs60681

      4 Luci/Gaussia Luciferase Reporter Gene Detection Kit

      500 & 2500 T

      Dual luciferase reporter gene detection new choice


      3. NanoLuc Luciferase Assay Reagents

      Abcam's NAG-L™ NanoLuc luciferase product series, developed based on HiBiT technology (a bioluminescence detection technology based on protein complementation), can detect intracellular or extracellular protein expression, degradation, and interactions. These products are characterized by high sensitivity and a high signal-to-noise ratio.


      Fig. 5. Schematic diagram of the lytic detection principle of NAG-L™ NanoLuc luciferase

      Product Number Product Name Specification Features

      abs60678

      NAG-L™ Lytic NanoLuc Luciferase Detection Kit 10 & 100 ml Lytic type, detects intracellular target proteins

      abs60679

      NAG-L™ Extracellular NanoLuc Luciferase Detection Kit 10 & 100 ml Non-lytic type, detects extracellular target proteins

      abs60680

      NAG-L™ Live Cell NanoLuc Luciferase Detection Kit 10 & 100 ml Substrate can enter cells, detects live cell target proteins

       

      4. Kinase Activity Assay Reagents

      Abcam's KNG-L™ series of kinase activity assay reagents utilize ATP-dependent luciferase luminescence reactions to quantitatively measure the consumption of ATP during the kinase catalysis process, thereby assessing the catalytic activity of kinases. The ADP-K™ kinase ADP detection kit, on the other hand, can assess kinase catalytic activity by measuring the content of ADP.





      Fig. 6. Schematic diagram of the detection principle of the ADP-K™ Kinase ADP Detection Kit


      Product Number Product Name Specification Features

      abs50194

      KNG-L™ Kinase Luminescence Detection Kit 10 & 100 ml Suitable for up to 10uM ATP system

      abs50195

      KNG-L™ Kinase Plus Luminescence Detection Kit 10 & 100 ml Suitable for up to 100uM ATP system

      abs50196

      KNG-L™ Kinase Max Luminescence Detection Kit 10 & 100 ml Suitable for up to 500uM ATP system

      abs50197

      ADP-K™ Kinase ADP Detection Kit 10 & 100 ml Suitable for up to 1mM ATP system

       

      5. Other Luminescence Detection Reagents

      Abcam also offers other detection reagents and consumables based on the principle of bioluminescence.

      Product Number Product Name Specification Features

      abs90283

      MYCO-L™ Luciferase Substrate Detection Kit 10 & 100 ml Detects substrate enzyme activity through luminescence, detects substrate

      abs42075819

      Luciferin Potassium Salt 25 & 100 mg Substrate for firefly luciferase, also used for small animal imaging

      abs42017259

      Luciferin Sodium Salt 25 & 100 mg Substrate for firefly luciferase, also used for small animal imaging

      abs7016

      Flat-bottom Luminescence Detection Microplate (96-well, white bottom, white lid) 50/box Luminescence detection experimental consumable

      abs7017

      Flat-bottom Luminescence Detection Microplate (96-well, black bottom, black lid) 50/box Luminescence detection experimental consumable

      abs7018

      Flat-bottom Luminescence Detection Microplate (96-well, white bottom, transparent lid) 50/box Luminescence detection experimental consumable

      abs7019

      Flat-bottom Luminescence Detection Microplate (96-well, black bottom, transparent lid) 50/box Luminescence detection experimental consumable

      abs7308

      Microplate (96-well, white, shallow well) 50/box Shallow well, suitable for small volume

      abs7311

      Microplate (384-well, white, shallow well) 40/box Shallow well, suitable for small volume

       

       

      Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.

      Absin Bioscience Inc.
      Email: worldwide@absin.net

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      June 11, 2025

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