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Two-Pronged Strategy: A Comprehensive Analysis of the Combined Application of mIHC and GeneScope Technologies
May 13, 2025
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In tumor immunology and precision medicine, integrating protein spatial localization with gene expression profiling has long been a daunting challenge. Absin pioneers a combined solution of GeneScope gene detection kits and multiplex fluorescence immunohistochemistry (mIHC) kits, offering a new paradigm for tumor microenvironment research, drug efficacy prediction, and clinical translation through technical synergy and data fusion.
Technical Synergy: Panoramic Protein-Gene Analysis
1. mIHC Kit: High-Sensitivity Protein Spatial Localization
Absin's mIHC kit, leveraging Tyramide Signal Amplification (TSA) technology, enables geometric amplification of fluorescence signals via HRP-mediated catalysis. This allows 2 - 9 - color multiplexing on a single slide, breaking the single-color limitation of traditional IHC. Its advantages are:
- High Sensitivity: Capable of detecting low-abundance targets like PD-1/PD-L1 and other immune checkpoint proteins.
- Multicolor Compatibility: Optimized dye combinations (e.g., TSA series) and staining sequences prevent spectral overlap, precisely displaying the spatial distribution of T cells, macrophages, etc., in the tumor microenvironment.
- Broad Sample Compatibility: Supports paraffin sections, frozen sections, and organoid co-culture models. Combined with antibody stripping buffer (abs994), it enables multiple staining cycles to expand research boundaries.
2. GeneScope: Gene Expression and Function Analysis
As Absin's spatial multi - omics platform, GeneScope integrates transcriptome sequencing and bioinformatics tools. It deeply explores gene expression networks related to mIHC - marked key proteins (e.g., Ki67, CD8 + T cells). For example:
- Tumor Heterogeneity Analysis: Combining mIHC protein spatial distribution with GeneScope gene mutation data reveals the molecular mechanisms of tertiary lymphoid structures (TLS) and recurrence - free survival (RFS).
- Drug Target Prediction: Joint analysis of PD - L1 protein expression and related signaling pathway genes (e.g., IFN - γ, JAK/STAT) optimizes immune therapy response prediction models.
Applications: From Basic Research to Clinical Translation
- Evaluation of Tumor Immunotherapy Efficacy
mIHC can detect immune checkpoint proteins like PD - 1, CTLA - 4, and FOXP3. GeneScope further analyzes T - cell exhaustion - related genes (e.g., TOX, TCF7), offering multidimensional evidence for combination therapies. Studies show this combined technology improves anti - PD - 1 therapy prediction accuracy by over 30% compared to traditional single - marker detection.
- Tumor Recurrence Risk Assessment
By marking tumor stem cell markers (e.g., CD133, ALDH1) via mIHC and analyzing stemness - related genes (e.g., OCT4, NANOG) with GeneScope, a recurrence risk stratification model can be built to guide post - operative monitoring strategies.
- Drug Development and Biomarker Discovery
In CAR - T therapy or nanomedicine research, the combined technology can track immune cell infiltration (e.g., CD8 + /CD4 + T - cell ratio) and key gene expression changes before and after treatment, accelerating candidate drug screening.
One - Stop Service Ecosystem: Accurate and Efficient Full - Process Support
Absin provides a complete ecosystem from experimental design to data mining:
- Pilot Experiment Optimization: Designs "bright - dim" dye strategies based on target abundance to avoid signal interference.
- Intelligent Analysis Tools: Integrates software like HALO and StrataQuest for quantitative cell subset analysis and spatial relationship visualization.
- Open Collaboration Network: Partners with research institutes and pharmaceutical companies to establish technology - application alliances, promoting the clinical diagnostic standardization of mIHC/GeneScope.
The combined application of Absin GeneScope and mIHC kits breaks down protein - gene research barriers, offering a comprehensive perspective on the tumor microenvironment from "spatial localization" to "molecular mechanisms." This technology duo is an indispensable tool for scientists exploring new immunotherapy targets or optimizing clinical treatment pathways.
Contact Absin now to unlock limitless multi - omics research possibilities!
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Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
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Absin 4-Color IHC Kit(Anti-Rabbit Secondary Antibody) |
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Antibody eluent (for mIHC) |
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Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.
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Absin Bioscience Inc. |
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