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Research on Molecular Interactions
April 25, 2025
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ChIP Technology
Chromatin Immunoprecipitation (ChIP) is a molecular biology technique used to study protein-DNA interactions. By utilizing specific antibodies against a known protein, the DNA interacting with the protein can be isolated from the complex. It is commonly used to determine the binding sites of specific transcription factors or other chromatin-associated proteins on the genome, as well as how these proteins regulate gene expression.
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Catalog Number |
Product Name |
Specifications |
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Chromatin Immunoprecipitation (ChIP) Kit |
22T |
Case Sharing:
Interleukin-34-orchestrated tumor-associated macrophage reprogramming is required for tumor immune escape driven by p53 inactivation
Immunity. 2024 Oct 8;57(10):2344-2361.e7. doi: 10.1016/j.immuni.2024.08.015. Epub 2024 Sep 24. PMID: 39321806 IF=25.5
To determine whether p53 binds to the regulatory region of IL-34, chromatin immunoprecipitation (ChIP) was performed using a p53 antibody, followed by high-throughput sequencing. Binding of p53 was observed at the IL34 and CDKN1A (p21) loci (Figure K). ChIP-PCR also revealed the binding of p53 to the IL34 promoter (Figure L).
DNA Pull Down
DNA pull-down is a molecular biology technique used to study protein-DNA interactions. Specific DNA probes targeting the region of interest are designed and labeled with biotin. The labeled probes can bind to streptavidin-conjugated magnetic beads. After incubation with total protein extracts, proteins that interact with the DNA probes can form magnetic bead-DNA probe-protein complexes. Non-specifically bound proteins are washed away, and the complexes are eluted. The interacting proteins are then identified by Western Blot or mass spectrometry (MS). This method is commonly used to identify transcription factors, DNA repair proteins, and other DNA-binding proteins.
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Catalog Number |
Product Name |
Specifications |
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DNA Pull Down Kit (Animal) |
6T |
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DNA Pull Down Kit (Plant and Bacteria) |
6T |
Case Sharing
Western Blot after DNA pull-down shows differential bands between control and experimental groups
RIP Technology
RNA Binding Protein Immunoprecipitation (RIP) is a technique used to study RNA-protein interactions within cells and is a powerful tool for understanding the dynamics of post-transcriptional regulatory networks. This method involves using specific antibodies against the target protein to precipitate RNA-protein complexes, followed by purification and analysis of the associated RNA by q-PCR or high-throughput sequencing.
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Catalog Number |
Product Name |
Specifications |
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RNA Immunoprecipitation (RIP) Kit |
6T/12T |
Case Sharing
Western Blot after RIP shows comparable results with imported products and differential bands compared to the IgG group
RNA Pull Down
RNA pull-down is a technique used to study RNA-protein interactions. RNA is first labeled (e.g., with biotin) and then incubated with cell lysates to form RNA-protein complexes. These complexes are subsequently detected for interacting proteins. After elution, the target RNA-protein interactions are identified by Western blot (pull-down-WB) and mass spectrometry (pull-down-MS).
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Catalog Number |
Product Name |
Specifications |
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RNA Pull Down Kit |
6T |
Case Sharing
Silver staining and enrichment detection by qPCR after pulldown
CoIP
Immunoprecipitation (IP/CoIP) is based on the specific affinity between antibodies and proteins. It captures target proteins and their interacting biomolecules from complex samples by using antibodies specific to the protein or antigen of interest.
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Catalog Number |
Product Name |
Specifications |
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Immunoprecipitation (IP/CoIP) Kit |
50T |
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Immunoprecipitation (IP/CoIP) Kit (Magnetic Bead Method) |
10T/50T |
Case Sharing
Targeted knockdown of PGAM5 in synovial macrophages efficiently alleviates osteoarthritis
Bone Res. 2024 Mar 4;12(1):15. doi: 10.1038/s41413-024-00318-8.PMID: 38433252 IF=12.7
Immunoprecipitation of DVL2 and PGAM5 in M1 and M2 macrophages (abs955)
GST Pull Down
The GST Pull-Down assay is based on the property of glutathione-S-transferase (GST), which can bind to glutathione (GSH). GSH is immobilized on magnetic beads to form GSH-beads. When a known protein X is fused with GST, the resulting GST-X protein can bind to the GSH-beads. If there is an interacting protein Y in the system, a complex of “bead-GSH-GST-X-Y” will form, allowing the interacting protein to be isolated and detected.
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Catalog Number |
Product Name |
Specifications |
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GST Pull Down Kit |
6T |
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Absin Bioscience Inc. |
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