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Decoding Immune Cell Communication: Multiplex Fluorescent Immunohistochemical Staining Technology
In the latest study, Reciprocal costimulatory molecules control the activation of mucosal type 3 innate lymphoid cells during engagement with B cells, the authors investigated how the activation of mucosal type 3 innate lymphoid cells (ILC3s) is controlled by reciprocal costimulatory molecules during their interaction with B cells. Scientists have unlocked a powerful visual tool that allows us to peek into the "whispers" between immune cells. This key is the multiplex immunofluorescence staining technique, which enables us to simultaneously observe the distribution of multiple biomarkers on the same tissue section.
Research Background:
ILC3s are a type of cell in the innate immune system with phenotypes similar to T helper cells and play a role in mucosal immunity;ICOS (Inducible T-cell costimulator) is a molecule expressed on T cells, involved in T cell activation and interactions between T cells and B cells in lymphoid tissues;The role of ICOS in ILC3s and its interactions with the immune microenvironment are still unclear.
Research Objectives:
To explore the expression of ICOS in ILC3s and its role in the interaction between ILC3s and the immune microenvironment;To investigate the specific role of ICOS signaling in the cross-talk between ILC3s and B cells.
In this study, scientists used the multiplex immunofluorescence staining kit from Absin (Catalog No.: abs50012), which acts like a magician's wand, making proteins within cells "glow" and revealing their precise locations within tissues. Researchers detected markers such as CD3, CD20, and RORγt. The results revealed the colocalization of ILC3s and B cells in tonsillar tissue and their interactions.
The Dance of Experimental Techniques
Researchers first processed tissue sections through a series of steps, including heating, dewaxing, dehydration, and antigen retrieval, followed by labeling target proteins with specific antibodies. These antibodies act like precise navigation systems, able to lock onto and identify specific cellular markers. Subsequently, with the aid of fluorescent dyes, these markers shine brightly under the microscope, like spotlights on a cellular stage, allowing scientists to clearly see the roles of each cell and their interactions.
The Brilliance of Multiplex Immunofluorescence Staining Technology
This technology not only allows us to observe the "dialogue" between cells but also reveals their complex interactions within the tumor microenvironment. It is like a meticulously choreographed dance, where each cell moves to its own rhythm, and multiplex immunofluorescence staining technology enables us to capture these movements and understand their coordination and rhythm.
In summary, through the use of multicolor techniques, researchers have drawn important conclusions about the molecular mechanisms of interaction between ILC3s and B cells, especially the crucial roles of ICOS and CD40 signaling in these interactions. These findings help us understand intercellular communication within the mucosal immune system and how to regulate these signaling pathways to treat related diseases. Multiplex immunofluorescence staining technology is like giving us a pair of X-ray eyes, allowing us to delve into the microscopic world of cells and explore their secrets. The application of this technology not only pushes the boundaries of scientific research but also provides new directions for future medical treatments. Let us look forward to these research findings being translated into powerful weapons against diseases as soon as possible!
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Absin 6-Color IHC Kit (Anti-Rabbit Secondary Antibody) |
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Absin 6-Color mlHC Kit(plus) (Anti-Rabbit Secondary Antibody) |
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Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
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Absin 5-Color IHC Kit (Anti-Rabbit Secondary Antibody) |
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Absin 4-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
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Absin 4-Color IHC Kit(Anti-Rabbit Secondary Antibody) |
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Antibody eluent (for mIHC) |
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Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.
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Absin Bioscience Inc. |
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April 21, 2025
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