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Absin Multiplex Fluorescence Immunohistochemistry: A Powerful Tool for C Research
【Introduction】
Chronic hepatitis B (CHB) represents a global health challenge, affecting hundreds of millions of individuals worldwide. To gain a comprehensive understanding of the complex mechanisms underlying this disease and to identify effective therapeutic approaches, scientists have been actively exploring innovative research tools and technologies. Recently, a groundbreaking study utilizing Absin's multiplex fluorescence immunohistochemistry technology has unveiled new insights into the etiology of intrahepatic inflammation in CHB.
【Research Content】
In this study, an international research team led by Dr. Jun Wang employed Absin's multiplex fluorescence immunohistochemistry kit (Catalog No. abs50029-20T) to conduct an in-depth analysis of liver samples from CHB patients. Through precise cell-type specific expression analysis in conjunction with clinical parameters, the research team explored the correlation between the liver transcriptome and intrahepatic inflammation in CHB.
【Multicolor Experimental Technology】
The Absin multiplex fluorescence immunohistochemistry technology utilized in this research is an advanced biomarker detection method that enables the simultaneous detection of multiple protein expressions and localization on a single tissue section. With this technology, researchers were able to precisely map the distribution of immune cells such as T cells, B cells, and macrophages within the liver, as well as their spatial association with HBV core antigen (HBcAg). Additionally, the study involved the detection of various immunomodulatory molecules and interferon-stimulated genes (ISGs), including TIGIT, CTLA4, CXCL8, CCL20, IFI44, IFI16, ISG15, and ISG20.
【Other Experimental Technologies】
Single-Cell RNA Sequencing (scRNA-seq): This technology was employed to analyze different cell types within the liver and identify specific marker genes for each cell type. Through this approach, researchers gained profound insights into cellular heterogeneity within the liver and classified cell populations across various clinical stages.
Population-Specific Expression Analysis (PSEA): This method decomposes bulk gene expression data into expression patterns specific to individual cell types. The PSEA model utilizes cell type marker genes from single-cell sequencing data to normalize the data, thereby estimating the relative abundance of gene expression within different cell populations.
Bayesian Linear Model: Researchers applied this model to link gene expression with clinical parameters, analyzing the roles of key factors such as inflammatory cells, immune activation, T cell exhaustion, chemokines, receptors, and interferon-stimulated genes (ISGs) across different clinical stages.
Multiplex Fluorescence Immunohistochemistry: This method was used to validate the results of multicolor immunohistochemistry in liver specimens, further confirming the expression patterns of key immune cells and molecules identified in the study.
Statistical and Bioinformatics Analyses: These included principal component analysis (PCA), gene set enrichment analysis (GSEA), hierarchical clustering analysis, and Bayesian statistical methods, which were utilized to process and interpret large-scale gene expression data and correlate this data with clinical parameters.
【Research Conclusion】
Through the application of Absin's multiplex fluorescence immunohistochemistry technology, the research team discovered increased gene expression related to immune cell activation and migration in CHB patients. Specifically, the expression of specific ISGs in T cells and macrophages was positively correlated with serum alanine aminotransferase (ALT) levels, rather than HBV DNA levels. This finding indicates that chemokines secreted by macrophages, such as CCL20 and CXCL8, play a crucial role in recruiting exhausted T cells into liver tissue. Simultaneously, the innate immune function of hepatocytes is suppressed, hindering the antiviral effects of ISGs.
【Significance】
This study not only provides novel insights into intrahepatic inflammation in chronic hepatitis B but also enables researchers to gain a deeper understanding of the relationship between immune cells and liver inflammation. It offers a scientific foundation for the development of new therapeutic strategies.
The application of Absin's multiplex fluorescence immunohistochemistry technology in this research once again demonstrates its value in biomedical research. With the continuous advancement of this advanced tool, we have reason to believe that future research on complex diseases such as chronic hepatitis B will become more in-depth, ultimately leading to more effective treatment options for patients.
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Catalog No. |
Product Name |
Specifications |
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Absin 7-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
20T/100T |
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Absin 7-Color IHC Kit(Anti-Rabbit Secondary Antibody) |
20T/100T |
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Absin 7-Color IHC Kit (plus) (Anti-Rabbit Secondary Antibody) |
20T/100T |
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Absin 6-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
20T/100T |
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Absin 6-Color IHC Kit (plus) (Anti-Rabbit and Mouse Secondary Antibody) |
20T/100T |
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Absin 6-Color IHC Kit (Anti-Rabbit Secondary Antibody) |
20T/100T |
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Absin 6-Color mlHC Kit(plus) (Anti-Rabbit Secondary Antibody) |
20T/100T |
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Absin 5-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
20T/100T |
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Absin 5-Color IHC Kit (Anti-Rabbit Secondary Antibody) |
20T/100T |
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Absin 4-Color IHC Kit (Anti-Rabbit and Mouse Secondary Antibody) |
20T/100T |
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Absin 4-Color IHC Kit(Anti-Rabbit Secondary Antibody) |
20T/100T |
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Antibody eluent (for mIHC) |
30mL |
Absin provides antibodies, proteins, ELISA kits, cell culture, detection kits, and other research reagents. If you have any product needs, please contact us.
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Absin Bioscience Inc. |
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April 18, 2025
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