What if the cells are contaminated with mycoplasma, call In Abigail

Mycoplasma is a prokaryote with a size of 0.2-0.3um and no cell wall, which can pass through the general filtration membrane (0.22-0.45um). In the process of cell culture, the infection rate of mycoplasma reaches 63%, which is a big headache in cell culture. Mycoplasma contamination, which may come from the medium, serum or the laboratory operator, can affect cell growth rate, cell morphology, gene expression, cell metabolism and cell viability. Mycoplasma infection is not easily detected, so it is very important to test the cultured cells for mycoplasma regularly.

So how do you know if the cells are contaminated with mycoplasma?

1. Changes in cell morphology
Mycoplasma contamination can be detected in turn by slower growth, significantly acidic pH in some media, and cytopathic appearance in severe cases. However, in some cases, the characteristics of the lesions caused by mycoplasma contamination are similar to that of viral infection, so an accurate diagnosis is not possible.

2. Kit test method:
The Albison Mycoplasma staining Kit can be used for in situ staining of mycoplasma or other prokaryotes in cultured cells. It is mainly used to detect the presence of mycoplasma contamination in cultured cells.
Bacterial contamination, yeast contamination, or mold contamination in cultured cells are all visible under light microscopy, but mycoplasma contamination is not visible under light microscopy and must be detected by specific detection methods.
There are many methods to detect mycoplasma contamination, including mycoplasma isolation and culture, mycoplasma specific enzyme detection, RT-PCR detection, and DNA fluorescence staining. The kit is designed to detect mycoplasma contamination using Hoechst staining, which is rapid, effective and highly sensitive for the detection of mycoplasma contamination.
If mycoplasma contamination is found, it is recommended that uncontaminated cells be replaced for culture. The kit can test at least 100 samples if used for six-well plate samples.

3. There are immovable small black spots in the intercellular space

How to prevent mycoplasma infection?

Regular testing: Mycoplasma infection will slowly wilt the cultured cells, so it is important to test the cultured cells regularly for mycoplasma. In general, testing for mycoplasma should be done every 1 to 3 months. The key for cell culture laboratory to deal with mycoplasma infection is to carry out regular mycoplasma testing. Use love believe mycoplasma prevention agent, can effectively eliminate serum, medium of the mycoplasma, the antibiotic: peptides, no resistance, no secondary pollution, as the extension of incubation time, will be a slow degradation of amino acids, can be used for large-scale fermentation, cell therapy, and other fields, and small cell toxicity, has been conducted on the many kinds of cells. Safe to use

If the cells are found to be infected, how to clear mycoplasma infection?

Mycoplasma contamination in cultured cells can be effectively inhibited and eliminated by albicium mycoplasma scavenging reagent. On the one hand, it can be used to eliminate the existing mycoplasma contamination in cells, but also can be used to prevent mycoplasma contamination. This reagent is a natural antibiotic obtained by screening. The concentration of inhibition or clearance of mycoplasma is relatively low and has no toxicity to cells. Unlike penicillin and streptomycin, which are commonly used in cell culture, there is no interference with various cell tests. Mycoplasma orale, M. Arginini, M. Hyorhinis and Acholeplasma Laidlawii are common laboratory Mycoplasma. Usually, adding this reagent to the cell culture medium for about a week can effectively inhibit or eliminate mycoplasma.

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